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Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza
Li, Bin1; Cui, Guanghong2; Shen, Guoan1; Zhan, Zhilai2; Huang, Luqi2; Chen, Jiachun; Qi, Xiaoquan1
2017
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号7
摘要CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/ Cas9 to precisely knock out the committed diterpene synthase gene ( SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacological activities, such as vasorelaxation, protection against ischemia-reperfusion injury, and antiarrhythmic effects. Three homozygous and eight chimeric mutants were obtained from 26 independent transgenic hairy root lines by Agrobacterium rhizogenes-mediated transformation. The metabolomic analysis based on LC-qTOF-MS and Q-TRAP-LC-MS/MS revealed that tanshinones, especially cryptotanshinone, tanshinone IIA and tanshinone I, are completely missing in homozygous mutants, without influencing other phenolic acid metabolites. By contrast, tanshinones are decreased but still detectable in chimeric mutants, which is similar to a previously-reported an RNAi study of SmCPS1. These results demonstrate that Agrobacterium rhizogenes-mediated transformation using CRISPR/Cas9 is a simple and efficient genome editing tool in S. miltiorrhiza, thus paving the way for large-scale genome editing in S. miltiorrhiza, which is important for pathway elucidation of secondary metabolites, quality improvement, and yield increases for this valuable traditional Chinese medicinal herb.
学科领域Plant Sciences ; Evolutionary Biology
DOI10.12705/663.3
收录类别SCI
语种英语
WOS关键词AGROBACTERIUM-RHIZOGENES ; HAIRY ROOT ; BIOSYNTHESIS ; TANSHINONES ; EXPRESSION ; BACTERIA ; PLASMID ; PATHWAY ; ARCHAEA ; SYSTEMS
WOS记录号WOS:000405738400003
出版者NATURE PUBLISHING GROUP
文献子类Article
出版地LONDON
资助机构Key Project of Chinese National Programs for Fundamental Research and Development [2013CB127000] ; Chinese National Programs for High Technology Research and Development [2012AA10A304]
作者邮箱homespringchen@126.com ; xqi@ibcas.ac.cn
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22367
专题中科院植物分子生理学重点实验室
作者单位1.Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式
GB/T 7714
Li, Bin,Cui, Guanghong,Shen, Guoan,et al. Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza[J]. SCIENTIFIC REPORTS,2017,7.
APA Li, Bin.,Cui, Guanghong.,Shen, Guoan.,Zhan, Zhilai.,Huang, Luqi.,...&Qi, Xiaoquan.(2017).Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza.SCIENTIFIC REPORTS,7.
MLA Li, Bin,et al."Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza".SCIENTIFIC REPORTS 7(2017).
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