Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice

doi:10.1105/tpc.16.00484

	Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice
	Yang, Ning; Wang, Tai
	2017
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	17
摘要	Background: The coordination of pollen tube (PT) growth, guidance and timely growth arrest and rupture mediated by PT-pistil interaction is crucial for the PT to transport sperm cells into ovules for double fertilization. The plasma membrane (PM) represents an important interface for cell-cell interaction, and PM proteins of PTs are pioneers for mediating PT integrity and interaction with pistils. Thus, understanding the mechanisms underlying these events is important for proteomics. Results: Using the efficient aqueous polymer two-phase system and alkali buffer treatment, we prepared high-purity PM from mature and germinated pollen of rice. We used iTRAQ quantitative proteomic methods and identified 1,121 PM-related proteins (PMrPs) (matched to 899 loci); 192 showed differential expression in the two pollen cell types, 119 increased and 73 decreased in abundance during germination. The PMrP and differentially expressed PMrP sets all showed a functional skew toward signal transduction, transporters, wall remodeling/metabolism and membrane trafficking. Their genomic loci had strong chromosome bias. We found 37 receptor-like kinases (RLKs) from 8 kinase subfamilies and 209 transporters involved in flux of diversified ions and metabolites. In combination with the rice pollen transcriptome data, we revealed that in general, the protein expression of these PMrPs disagreed with their mRNA expression, with inconsistent mRNA expression for 74% of differentially expressed PMrPs. Conclusions: This study identified genome-wide pollen PMrPs, and provided insights into the membrane profile of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils. These pollen PMrPs and their mRNAs showed discordant expression. This work provides resource and knowledge to further dissect mechanisms by which pollen or the PT controls PMrP abundance and monitors interactions and ion and metabolite exchanges with female cells in rice.
关键词	Plasma membrane Receptor-like kinase Transporters Quantitative proteomics iTRAQ Pollen-pistil interaction Rice
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.16.00484
收录类别	SCI
语种	英语
WOS关键词	ORYZA-SATIVA L. ; MONOSACCHARIDE TRANSPORTER ; ARABIDOPSIS-THALIANA ; BRASSICA-OLERACEA ; MOLECULAR-CLONING ; DRAFT SEQUENCE ; TIP GROWTH ; GENE ; CELL ; CHANNEL
WOS记录号	WOS:000404989600015
出版者	BIOMED CENTRAL LTD
文献子类	Article
出版地	LONDON
资助机构	Ministry of Science and TechnologyMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) [2013CB945101, 2012CB910504]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22370
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China; Natl Ctr Plant Gene Res, 20 Nanxincun, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yang, Ning,Wang, Tai. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice[J]. BMC PLANT BIOLOGY,2017,17.
APA	Yang, Ning,&Wang, Tai.(2017).Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice.BMC PLANT BIOLOGY,17.
MLA	Yang, Ning,et al."Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice".BMC PLANT BIOLOGY 17(2017).

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