Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli

doi:10.1038/srep19467

	Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli
	Gao, Jie ; Lan, Ting 1
	2016
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	Late embryogenesis abundant (LEA) proteins are a large and highly diverse gene family present in a wide range of plant species. LEAs are proposed to play a role in various stress tolerance responses. Our study represents the first-ever survey of LEA proteins and their encoding genes in a widely distributed pine (Pinus tabuliformis) in China. Twenty-three LEA genes were identified from the P. tabuliformis belonging to seven groups. Proteins with repeated motifs are an important feature specific to LEA groups. Ten of 23 pine LEA genes were selectively expressed in specific tissues, and showed expression divergence within each group. In addition, we selected 13 genes representing each group and introduced theses genes into Escherichia coli to assess the protective function of PtaLEA under heat and salt stresses. Compared with control cells, the E. coli cells expressing PtaLEA fusion protein exhibited enhanced salt and heat resistance and viability, indicating the protein may play a protective role in cells under stress conditions. Furthermore, among these enhanced tolerance genes, a certain extent of function divergence appeared within a gene group as well as between gene groups, suggesting potential functional diversity of this gene family in conifers.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep19467
收录类别	SCI
语种	英语
WOS关键词	SACCHAROMYCES-CEREVISIAE ; ABSCISIC-ACID ; SALT STRESS ; MAIZE DHN1 ; TOLERANCE ; EXPRESSION ; IDENTIFICATION ; DROUGHT ; BINDING ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000368291900001
出版者	NATURE RESEARCH
文献子类	Article
出版地	BERLIN
资助机构	National Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31300560]
作者邮箱	gaojie@xtbg.org.cn
作品OA属性	Green Published, gold
引用统计	被引频次：79[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24943
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Menglun, Yunnan, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systmat & Evolutionary Bot, Beijing 10093, Peoples R China
推荐引用方式 GB/T 7714	Gao, Jie,Lan, Ting. Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli[J]. SCIENTIFIC REPORTS,2016,6.
APA	Gao, Jie,&Lan, Ting.(2016).Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli.SCIENTIFIC REPORTS,6.
MLA	Gao, Jie,et al."Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli".SCIENTIFIC REPORTS 6(2016).

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