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MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.) | |
Li, Zhe; Zhen, Zhen1; Guo, Kai; Harvey, Paul2; Li, Jishun; Yang, Hetong | |
2016 | |
发表期刊 | PROTOPLASMA |
ISSN | 0033-183X |
卷号 | 253期号:2页码:553-569 |
摘要 | Vacuolar H+-ATPase (V-H+-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H+-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint (Mentha x piperita L.) Keyuan-1 treated with different concentrations of NaCl. Results showed that the expressions of V-H+-ATPase in the transcriptional, protein and activity levels were significantly enhanced in the halotolerate peppermint Keyuan-1 compared to the wild-type (WT) peppermint under 50, 100, and 150 mM NaCl treatment. Moreover, inhibition experiments exhibited that V-H+-ATPase activity played vital roles in the salt tolerance of peppermint Keyuan-1 to 150 mM NaCl stress through increasing the vacuolar H+ pumping activity and Na+ compartmentalization capacity. Furthermore, results of Western blots showed that the activity of a mitogen-activated protein kinase (MAPK) was significantly increased under different concentrations of NaCl in the halotolerate peppermint Keyuan-1, which was much higher than that of WT peppermint. Further experiments with inhibitors suggested that this MAPK protein was responsible for the enhanced expression of V-H+-ATPase in the halotolerate peppermint Keyuan-1. In response to NaCl stress, increase of cytoplasmic calcium concentration ([Ca2+](cyt)) occurred upstream of MAPK activation in the halotolerate peppermint Keyuan-1. In all, these findings demonstrated that increased V-H+-ATPase activity was positively correlated with the enhanced salt tolerance in the halotolerate peppermint Keyuan-1, providing the theoretic basis for the further development and utilization of peppermint in saline areas. |
关键词 | Vacuolar H+-ATPase H+ pumping activity Na+ compartmentalization MAPK cascade Calcium Peppermint |
学科领域 | Plant Sciences ; Cell Biology |
DOI | 10.1007/s00709-015-0834-1 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | PYROPHOSPHATASE ACTIVITIES ; ALUMINUM PHYTOTOXICITY ; NA+/H+ ANTIPORTER ; PROTEIN-KINASE ; SALT TOLERANCE ; CALCIUM ; FLUORESCENCE ; TRANSPORT ; DROUGHT ; GROWTH |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000373578100028 |
出版者 | SPRINGER WIEN |
文献子类 | Article |
出版地 | WIEN |
EISSN | 1615-6102 |
资助机构 | Programs for International S&T Cooperation Program of China [2011DFA30990, 2007DFA30630] ; Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [2014ZRC01073, ZR2014YL002] ; Youth Science Funds of Shandong Academy of Sciences [2013QN012, 2014QN017, 2014QN019] ; Taishan Scholars Program for Overseas Experts of Shandong Province ; National High Technology Research and Development Program (863 Program) of ChinaNational High Technology Research and Development Program of China [2011AA10A205] ; Special Foundation for the Basic Work of Science and Technology [2014FY120900] ; Special Fund for Agro-scientific Research in the Public Interest [201303025] |
作者邮箱 | lizh@sdas.org |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/24948 |
专题 | 植被与环境变化国家重点实验室 |
作者单位 | 1.Shandong Acad Sci, Ctr Biotechnol, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Commonwealth Sci & Ind Res Org, Div Ecosyst Sci, Clayton, Vic 3169, Australia |
推荐引用方式 GB/T 7714 | Li, Zhe,Zhen, Zhen,Guo, Kai,et al. MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)[J]. PROTOPLASMA,2016,253(2):553-569. |
APA | Li, Zhe,Zhen, Zhen,Guo, Kai,Harvey, Paul,Li, Jishun,&Yang, Hetong.(2016).MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.).PROTOPLASMA,253(2),553-569. |
MLA | Li, Zhe,et al."MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)".PROTOPLASMA 253.2(2016):553-569. |
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