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CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis
Li, Shibai; Wang, Xiaochen2; He, Shan; Li, Jieru; Huang, Qingpei; Imaizumi, Takato3; Qu, Leqing2; Qin, Genji; Qu, Li-Jia; Gu, Hongya4
2016
发表期刊PLOS GENETICS
ISSN1553-7404
卷号12期号:1
摘要The cuticle is a hydrophobic lipid layer covering the epidermal cells of terrestrial plants. Although many genes involved in Arabidopsis cuticle development have been identified, the transcriptional regulation of these genes is largely unknown. Previously, we demonstrated that AtCFL1 negatively regulates cuticle development by interacting with the HD-ZIP IV transcription factor HDG1. Here, we report that two bHLH transcription factors, AtCFL1 associated protein 1 (CFLAP1) and CFLAP2, are also involved in AtCFL1-mediated regulation of cuticle development. CFLAP1 and CFLAP2 interact with AtCFL1 both in vitro and in vivo. Overexpression of either CFLAP1 or CFLAP2 led to expressional changes of genes involved in fatty acids, cutin and wax biosynthesis pathways and caused multiple cuticle defective phenotypes such as organ fusion, breakage of the cuticle layer and decreased epicuticular wax crystal loading. Functional inactivation of CFLAP1 and CFLAP2 by chimeric repression technology caused opposite phenotypes to the CFLAP1 overexpressor plants. Interestingly, we find that, similar to the transcription factor HDG1, the function of CFLAP1 in cuticle development is dependent on the presence of AtCFL1. Furthermore, both HDG1 and CFLAP1/2 interact with the same C-terminal C4 zinc finger domain of AtCFL1, a domain that is essential for AtCFL1 function. These results suggest that AtCFL1 may serve as a master regulator in the transcriptional regulation of cuticle development, and that CFLAP1 and CFLAP2 are involved in the AtCFL1-mediated regulation pathway, probably through competing with HDG1 to bind to AtCFL1.
学科领域Genetics & Heredity
DOI10.1371/journal.pgen.1005744
收录类别SCI
语种英语
WOS关键词CUTICULAR WAX BIOSYNTHESIS ; FATTY-ACID ELONGASE ; DROUGHT TOLERANCE ; PROTEIN INTERACTIONS ; OMEGA-HYDROXYLATION ; FUNCTIONAL-ANALYSIS ; CONDENSING ENZYME ; CELL-INTERACTIONS ; FACTOR GENE ; ENCODES
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000369368200005
出版者PUBLIC LIBRARY SCIENCE
文献子类Article
出版地SAN FRANCISCO
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91331201] ; Ministry of Education of the People's Republic of ChinaMinistry of Education, China [B06001] ; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [GM079712] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM079712] Funding Source: NIH RePORTER
作者邮箱guhy@pku.edu.cn
作品OA属性Green Submitted, Green Published, gold
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/24949
专题中科院植物分子生理学重点实验室
作者单位1.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
2.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing, Peoples R China
4.Univ Washington, Dept Biol, Seattle, WA 98195 USA
5.Natl Plant Gene Res Ctr Beijing, Beijing, Peoples R China
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GB/T 7714
Li, Shibai,Wang, Xiaochen,He, Shan,et al. CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis[J]. PLOS GENETICS,2016,12(1).
APA Li, Shibai.,Wang, Xiaochen.,He, Shan.,Li, Jieru.,Huang, Qingpei.,...&Gu, Hongya.(2016).CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis.PLOS GENETICS,12(1).
MLA Li, Shibai,et al."CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis".PLOS GENETICS 12.1(2016).
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