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| CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis | |
Li, Shibai; Wang, Xiaochen2; He, Shan; Li, Jieru; Huang, Qingpei; Imaizumi, Takato3; Qu, Leqing2 ; Qin, Genji; Qu, Li-Jia; Gu, Hongya4
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| 2016 | |
| Source Publication | PLOS GENETICS
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| ISSN | 1553-7404 |
| Volume | 12Issue:1 |
| Abstract | The cuticle is a hydrophobic lipid layer covering the epidermal cells of terrestrial plants. Although many genes involved in Arabidopsis cuticle development have been identified, the transcriptional regulation of these genes is largely unknown. Previously, we demonstrated that AtCFL1 negatively regulates cuticle development by interacting with the HD-ZIP IV transcription factor HDG1. Here, we report that two bHLH transcription factors, AtCFL1 associated protein 1 (CFLAP1) and CFLAP2, are also involved in AtCFL1-mediated regulation of cuticle development. CFLAP1 and CFLAP2 interact with AtCFL1 both in vitro and in vivo. Overexpression of either CFLAP1 or CFLAP2 led to expressional changes of genes involved in fatty acids, cutin and wax biosynthesis pathways and caused multiple cuticle defective phenotypes such as organ fusion, breakage of the cuticle layer and decreased epicuticular wax crystal loading. Functional inactivation of CFLAP1 and CFLAP2 by chimeric repression technology caused opposite phenotypes to the CFLAP1 overexpressor plants. Interestingly, we find that, similar to the transcription factor HDG1, the function of CFLAP1 in cuticle development is dependent on the presence of AtCFL1. Furthermore, both HDG1 and CFLAP1/2 interact with the same C-terminal C4 zinc finger domain of AtCFL1, a domain that is essential for AtCFL1 function. These results suggest that AtCFL1 may serve as a master regulator in the transcriptional regulation of cuticle development, and that CFLAP1 and CFLAP2 are involved in the AtCFL1-mediated regulation pathway, probably through competing with HDG1 to bind to AtCFL1. |
| Subject Area | Genetics & Heredity |
| DOI | 10.1371/journal.pgen.1005744 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CUTICULAR WAX BIOSYNTHESIS ; FATTY-ACID ELONGASE ; DROUGHT TOLERANCE ; PROTEIN INTERACTIONS ; OMEGA-HYDROXYLATION ; FUNCTIONAL-ANALYSIS ; CONDENSING ENZYME ; CELL-INTERACTIONS ; FACTOR GENE ; ENCODES |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000369368200005 |
| Publisher | PUBLIC LIBRARY SCIENCE |
| Subtype | Article |
| Publication Place | SAN FRANCISCO |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91331201] ; Ministry of Education of the People's Republic of ChinaMinistry of Education, China [B06001] ; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [GM079712] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM079712] Funding Source: NIH RePORTER |
| Corresponding Author Email | guhy@pku.edu.cn |
| OA | Green Submitted, Green Published, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/24949 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 2.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing, Peoples R China 4.Univ Washington, Dept Biol, Seattle, WA 98195 USA 5.Natl Plant Gene Res Ctr Beijing, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Shibai,Wang, Xiaochen,He, Shan,et al. CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis[J]. PLOS GENETICS,2016,12(1). |
| APA | Li, Shibai.,Wang, Xiaochen.,He, Shan.,Li, Jieru.,Huang, Qingpei.,...&Gu, Hongya.(2016).CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis.PLOS GENETICS,12(1). |
| MLA | Li, Shibai,et al."CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis".PLOS GENETICS 12.1(2016). |
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| File Name/Size | DocType | Version | Access | License | ||
| Li-2016-CFLAP1 and C（4079KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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