Fast identification of the antibacterial in Aspergillus species

doi:10.18054/pb.v118i4.4155

	Fast identification of the antibacterial in Aspergillus species
	Wang, Xiaojing ; Duan, Lixin 1; Yang, Shengnan ; Zhou, Zhengfu ; Dun, Baoqing 2
	2016
发表期刊	PERIODICUM BIOLOGORUM
ISSN	0031-5362
卷号	118 期号:4 页码:425-430
摘要	Background and Purpose: Aspergillus genus biosynthesize a large number of metabolites with wide-ranging bioactivities, but how to target the active compounds quickly and accurately? In this work, we provide a strategy to fast identify bioactive metabolites in fungi with similar morphology but different antimicrobial activities. Materials and Methods: Combined with High Performance Liquid Chromatography (HPLC) profiles and bioactivity screening of fungal extracts, three Aspergillus sp. strains YSN038, YSN052 and YSN064 were studied. The bacteria Bacillus pumilus, Bacillus subtilis, Staphylococcus aureus, Kocuria rhizophila, Escherichia coli and Ralstonia solanacearum were used for antibiotic assay. Results: Three strains showed the similar morphology, but the crude extracts of YSN038 and YSN064 displayed antibacterial activities against B. pumilus, B. subtilis, S. aureus and K. rhizophila, whereas YSN052 had not. The compound contributed to antibacterial activity in vitro was rapidly identified, isolated and characterized. Compound 3 connected with specific HPLC peak should be the antibiotic substance by analysis of HPLC profiles, and was confirmed after the following antimicrobial tests. The compound was elucidated as butyrolactone I based on nuclear magnetic resonance(NMR) and mass spectrometry (MS) data, and showed various biological activities. Conclusions: Our study has significant scope on targeting antibacterial metabolites. The described method could also be used as a rapid and cost-effective tool for screening other bioactivity products. As such, this article could offer a fast approach to isolate drug-lead compounds from microorganisms.
关键词	Antibacterial activity HPLC Aspergillus Bioactive metabolites
学科领域	Biology
DOI	10.18054/pb.v118i4.4155
收录类别	SCI
语种	英语
WOS关键词	BUTYROLACTONE-I DERIVATIVES ; BOVINE OOCYTES ; TERREUS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000397425700013
出版者	PERIODICUM BIOLOGORUM
文献子类	Article
出版地	ZAGREB
资助机构	National Basic Research (973) Program of ChinaNational Basic Research Program of China [2015CB755703] ; 948 program of Ministry of Agriculture of China [2016-X43]
作者邮箱	dunbaoqing@caas.cn
作品OA属性	Green Published, gold
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24954
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaojing,Duan, Lixin,Yang, Shengnan,et al. Fast identification of the antibacterial in Aspergillus species[J]. PERIODICUM BIOLOGORUM,2016,118(4):425-430.
APA	Wang, Xiaojing,Duan, Lixin,Yang, Shengnan,Zhou, Zhengfu,&Dun, Baoqing.(2016).Fast identification of the antibacterial in Aspergillus species.PERIODICUM BIOLOGORUM,118(4),425-430.
MLA	Wang, Xiaojing,et al."Fast identification of the antibacterial in Aspergillus species".PERIODICUM BIOLOGORUM 118.4(2016):425-430.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Wang-2016-Fast ident（2358KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文