Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient?

doi:10.1093/jpe/rtv084

	Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient?
	Yuan, Shan 1; Guo, Chengyuan ; Ma, Linna ; Wang, Renzhong
	2016
发表期刊	JOURNAL OF PLANT ECOLOGY
ISSN	1752-9921
卷号	9 期号:5 页码:616-628
摘要	The two coexisting Leymus chinensis ecotypes exhibit remarkable divergences in adaptive strategies under drought and salinity in semi-humid meadows and semi-arid steppes. In order to detect the major genetic and environmental factors dominating the intraspecific phenotype variations and ecotype formation, the questions regarding the two distinct phenotypic forms (ecotypes) in L. chinensis were addressed: (i) did environments drive the L. chinensis ecotype formation? (ii) was there a molecular basis for the morphological divergence between the two ecotypes? (iii) which driving force dominated the intraspecies divergence, divergent natural selection, genetic drift or stabilizing selection? We applied a series experiments on demographical, morphological and physiological traits of two Leymus chinensis ecotypes with gray green (GG) and yellow green (YG) leaf color in nine wild sites along a longitudinal gradient from 114A degrees to 124A degrees E in northeast China. The environmental data including mean annual precipitation, mean annual temperature, elevation and soil properties were collected. We compared the differences of morphological, physiological and genetic differentiations between the two ecotypes. The GG type exhibited stronger fitness than YG type from the population densities, morphological traits (e.g. shoot height, leaf area, leaf and seed weights et al.), leaf mass per area (LMA) and physiological traits [relative water content (RWC), proline, soluble sugar contents]. Most of above phenotypes (e.g. total shoot densities, spike length et al.) were significantly correlated with mean annual precipitation, mean annual temperature and soil water content (SWC), rarely a correlated with soil pH and soil nutrient. Transplanted populations showed convergence trend by their leaf chlorophyll contents and osmotic adjustments (proline and soluble sugar contents) in the greenhouse, but still exhibited their divergences between two ecotypes in the outdoor transplantation, suggesting that whether L. chinensis ecotype differentiated could be largely affected by the environmental conditions. Furthermore, by the comparison result of quantitative genetic variation (Q (ST)) values from phenotypes with theoretical neutral genetic differentiation (F (ST)), differentiation in phenotypic traits greatly surpassed neutral predictions, implying that directional natural selection played a crucial role in L. chinensis ecotype differentiation. In addition, microsatellite analysis from Neighbor-joining tree and Bayesian assignment generated into two groups according to ecotypes, indicating molecular genetic differentiation also propelled the two ecotypes divergence. We conclude that L. chinensis population variations were driven by combing divergent natural selection (precipitation, temperature and SWCs) along the large-scale gradient and significantly intrinsic genetic differentiation.
关键词	genetic differentiation large-scale gradient microsatellites phenotypic traits Leymus chinensis ecotypes
学科领域	Plant Sciences ; Ecology ; Forestry
DOI	10.1093/jpe/rtv084
收录类别	SCI
语种	英语
WOS关键词	F-ST ; PHENOTYPIC PLASTICITY ; POPULATION-STRUCTURE ; ARABIDOPSIS-THALIANA ; NORTHEAST CHINA ; PLANT ; SPECIATION ; TRITICEAE ; DISTANCE ; Q(ST)
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000386153600013
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1752-993X
资助机构	National Scientific Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470404, 31070228]
作者邮箱	wangrz@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24963
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Shan,Guo, Chengyuan,Ma, Linna,et al. Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient?[J]. JOURNAL OF PLANT ECOLOGY,2016,9(5):616-628.
APA	Yuan, Shan,Guo, Chengyuan,Ma, Linna,&Wang, Renzhong.(2016).Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient?.JOURNAL OF PLANT ECOLOGY,9(5),616-628.
MLA	Yuan, Shan,et al."Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient?".JOURNAL OF PLANT ECOLOGY 9.5(2016):616-628.

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