Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex

doi:10.1104/pp.15.01893

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	Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex
	Shapiguzov, Alexey 1,3,4; Chai, Xin ; Fucile, Geoffrey ; Longoni, Paolo ; Zhang, Lixin; Rochaix, Jean-David
	2016
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	171 期号:1 页码:82-92
摘要	Photosynthetic organisms have the ability to adapt to changes in light quality by readjusting the cross sections of the light-harvesting systems of photosystem II (PSII) and photosystem I (PSI). This process, called state transitions, maintains the redox poise of the photosynthetic electron transfer chain and ensures a high photosynthetic yield when light is limiting. It is mediated by the Stt7/STN7 protein kinase, which is activated through the cytochrome b(6)f complex upon reduction of the plastoquinone pool. Its probable major substrate, the light-harvesting complex of PSII, once phosphorylated, dissociates from PSII and docks to PSI, thereby restoring the balance of absorbed light excitation energy between the two photosystems. Although the kinase is known to be inactivated under high-light intensities, the molecular mechanisms governing its regulation remain unknown. In this study we monitored the redox state of a conserved and essential Cys pair of the Stt7/STN7 kinase and show that it forms a disulfide bridge. We could not detect any change in the redox state of these Cys during state transitions and high-light treatment. It is only after prolonged anaerobiosis that this disulfide bridge is reduced. It is likely to be mainly intramolecular, although kinase activation may involve a transient covalently linked kinase dimer with two intermolecular disulfide bonds. Using the yeast two-hybrid system, we have mapped one interaction site of the kinase on the Rieske protein of the cytochrome b(6)f complex.
学科领域	Plant Sciences
DOI	10.1104/pp.15.01893
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYSTEM-II ; STATE TRANSITIONS ; PROTEIN-PHOSPHORYLATION ; ELECTRON-TRANSFER ; LIGHT ; LHCII ; PHOTOSYNTHESIS ; PLASTOQUINOL ; CHLOROPLASTS ; FLUORESCENCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000375431500006
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Swiss National FoundationSwiss National Science Foundation (SNSF) [3100AO-117712] ; Foreign Expert Program of the Chinese Academy of Sciences
作者邮箱	jean-david.rochaix@unige.ch
作品OA属性	Bronze, Green Published
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24975
专题	中科院光生物学重点实验室
作者单位	1.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland 2.Univ Geneva, Dept Plant Biol, Geneva, Switzerland 3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing, Peoples R China 4.Russian Acad Sci, Inst Plant Physiol, Bot Skaya St 35, Moscow 127276, Russia 5.Univ Helsinki, Dept Biosci, Div Plant Biol, Viikki Plant Sci Ctr, FI-00014 Helsinki, Finland
推荐引用方式 GB/T 7714	Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,et al. Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex[J]. PLANT PHYSIOLOGY,2016,171(1):82-92.
APA	Shapiguzov, Alexey,Chai, Xin,Fucile, Geoffrey,Longoni, Paolo,Zhang, Lixin,&Rochaix, Jean-David.(2016).Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex.PLANT PHYSIOLOGY,171(1),82-92.
MLA	Shapiguzov, Alexey,et al."Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b(6)f Complex".PLANT PHYSIOLOGY 171.1(2016):82-92.

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