Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp

doi:10.1007/s10811-015-0607-4

IB-CAS > 中科院光生物学重点实验室

	Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp
	Jia, Xiao-Hui ; Zhang, Chun-Li ; Shi, Ding-Ji ; Zhuang, Min-Min ; Wang, Xiang ; Jia, Rui ; Zhang, Zheng-Yang ; Huang, Jie 3; Sun, Yi-Hua ; Qian, Wen-Yi ; Peng, Guo-Hong ; He, Pei-Min
	2016
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	28 期号:2 页码:1001-1009
摘要	White spot syndrome virus (WSSV) is one of the most prevalent and lethal viruses for shrimp, and oral administration of VP28 is a promising approach to protect shrimp against WSSV. Although seven heterologous expression systems have been successful since 2004, no one has been commercialized in shrimp industry. This paper presented the idea of both drug and feed from the same feedstock to develop oral vaccine against WSSV. Filamentous, heterocystous cyanobacterium, Anabaena sp. PCC 7120, was used to express major envelope protein, VP28, for feeding post larvae of shrimp. The expression of vp28 gene in transgenic mutant was analyzed by Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). For vaccination, Litopenaeus vannamei was orally administrated by feeding the wild type and mutants of Anabaena cells for 10 days, and was challenged with WSSV during 10 days post vaccination. The post larvae were fed with the mutant harboring vp28 (10 mu g shrimp(-1)). The post larvae were divided five groups and their survivals were as follows: (1) negative control (neither vaccination nor challenge), 100 %; (2) positive control (challenge and no vaccination), 0 %; (3) feeding the wild type, 15.5 %; (4) feeding blank, 20.6 %; and (5) feeding the mutant harboring vp28, 68.0 %. Moreover, the post larvae fed the mutant harboring vp28 grew better than no feed. Also, the photosynthetic features indicated the transgenic mutant was easier to cultivate. These data show that transgenic mutant harboring vp28 was favorable not only to use both for drug and feed but also to cultivate in scale.
关键词	Anabaena sp PCC 7120 Cyanobacteria White spot syndrome virus (WSSV) vp28 gene expression Oral vaccine Photosynthesis
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-015-0607-4
收录类别	SCI
语种	英语
WOS关键词	MAJOR ENVELOPE PROTEINS ; BACILLUS-SUBTILIS ; ENGINEERING CYANOBACTERIA ; LITOPENAEUS-VANNAMEI ; PROCAMBARUS-CLARKII ; VACCINATION TRIALS ; PENAEUS-MONODON ; GENE-EXPRESSION ; WSSV INFECTION ; PROTECTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000372256900029
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5176
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2014AA093506] ; Shanghai Science and Technology CommissionShanghai Science & Technology CommitteeScience & Technology Commission of Shanghai Municipality (STCSM) [12391901700] ; Shanghai Universities First-class Disciplines Project, Discipline name, Marine Science [0707]
作者邮箱	cyanoshi@163.com ; rjia@shou.edu.cn ; pmhe@shou.edu.cn
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24977
专题	中科院光生物学重点实验室
作者单位	1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ Shanghai, Shanghai 201306, Peoples R China 2.Max Planck Inst Biophys, Dept Mol Membrane Biol, Max von Laue Str 3, D-60438 Frankfurt, Germany 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Jia, Xiao-Hui,Zhang, Chun-Li,Shi, Ding-Ji,et al. Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(2):1001-1009.
APA	Jia, Xiao-Hui.,Zhang, Chun-Li.,Shi, Ding-Ji.,Zhuang, Min-Min.,Wang, Xiang.,...&He, Pei-Min.(2016).Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp.JOURNAL OF APPLIED PHYCOLOGY,28(2),1001-1009.
MLA	Jia, Xiao-Hui,et al."Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp".JOURNAL OF APPLIED PHYCOLOGY 28.2(2016):1001-1009.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Jia-2016-Oral admini（1588KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文