Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves

doi:10.3390/ijms17030346

	Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves
	Yu, Juanjuan 1; Zhang, Jinzheng 2; Zhao, Qi ; Liu, Yuelu 2; Chen, Sixue 3; Guo, Hongliang 4; Shi, Lei2 ; Dai, Shaojun
	2016
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	17 期号:3
摘要	Leaf color change of variegated leaves from chimera species is regulated by fine-tuned molecular mechanisms. Hosta Gold Standard is a typical chimera Hosta species with golden-green variegated leaves, which is an ideal material to investigate the molecular mechanisms of leaf variegation. In this study, the margin and center regions of young and mature leaves from Hosta Gold Standard, as well as the leaves from plants after excess nitrogen fertilization were studied using physiological and comparative proteomic approaches. We identified 31 differentially expressed proteins in various regions and development stages of variegated leaves. Some of them may be related to the leaf color regulation in Hosta Gold Standard. For example, cytosolic glutamine synthetase (GS1), heat shock protein 70 (Hsp70), and chloroplastic elongation factor G (cpEF-G) were involved in pigment-related nitrogen synthesis as well as protein synthesis and processing. By integrating the proteomics data with physiological results, we revealed the metabolic patterns of nitrogen metabolism, photosynthesis, energy supply, as well as chloroplast protein synthesis, import and processing in various leaf regions at different development stages. Additionally, chloroplast-localized proteoforms involved in nitrogen metabolism, photosynthesis and protein processing implied that post-translational modifications were crucial for leaf color regulation. These results provide new clues toward understanding the mechanisms of leaf color regulation in variegated leaves.
关键词	variegated leaves Hosta Gold Standard excess nitrogen fertilization proteomics leaf color
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms17030346
收录类别	SCI
语种	英语
WOS关键词	CYTOSOLIC GLUTAMINE-SYNTHETASE ; DEFICIENT AUREA MUTANT ; VARIEGATED LEAVES ; BLUE-LIGHT ; CHLOROPLAST-BIOGENESIS ; NITROGEN ASSIMILATION ; MOLECULAR ANALYSIS ; PROTEIN IMPORT ; NUCLEAR GENES ; HIGHER-PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373712800047
出版者	MDPI
文献子类	Article
出版地	BASEL
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270310] ; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning ; Capacity Construction Project of Local Universities, Shanghai, China [14390502700] ; Knowledge Innovation Program of the Chinese Academy of ScienceKnowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-B-05, KSCX2-EW-B-02] ; Science and Technology Basic Work of Science and Technology [2014F210300] ; Funding Program for Young Teachers at Universities and Colleges of Shanghai ; General Scientific Research Project of Shanghai Normal University [SK201419]
作者邮箱	yujuan8186@163.com ; caohua@ibcas.ac.cn ; zhaoqizq@yeah.net ; liuyuelu09@sina.com ; schen@ufl.edu ; guohongliang97@126.com ; shilei@ibcas.ac.cn ; daishaojun@hotmail.com
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24980
专题	中科院北方资源植物重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Univ Florida, Genet Inst, Dept Biol, Interdisciplinary Ctr Biotechnol Res,Plant Mol &, Gainesville, FL 32610 USA 5.Harbin Univ Commerce, Food Engn Coll, Harbin 150028, Peoples R China
推荐引用方式 GB/T 7714	Yu, Juanjuan,Zhang, Jinzheng,Zhao, Qi,et al. Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3).
APA	Yu, Juanjuan.,Zhang, Jinzheng.,Zhao, Qi.,Liu, Yuelu.,Chen, Sixue.,...&Dai, Shaojun.(2016).Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(3).
MLA	Yu, Juanjuan,et al."Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.3(2016).

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