Proteome analysis of alfalfa roots in response to water deficit stress

doi:10.1016/S2095-3119(15)61255-2

	Proteome analysis of alfalfa roots in response to water deficit stress
	Atikur, Rahman Md ; Yong-goo, Kim ; Iftekhar, Alam 2; Liu Gong-she; Hyoshin, Lee 3; Joo, Lee Jeung 4; Byung-Hyun, Lee
	2016
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	15 期号:6 页码:1275-1285
摘要	To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H2O2 content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.
关键词	alfalfa proteome root water deficit stress
学科领域	Agriculture, Multidisciplinary
DOI	10.1016/S2095-3119(15)61255-2
收录类别	SCI
语种	英语
WOS关键词	TERM DROUGHT ; SYNTHETASE ; PROTEINS ; OVEREXPRESSION ; TOLERANCE ; PLANT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000378188600010
出版者	ELSEVIER SCI LTD
文献子类	Article
出版地	OXFORD
资助机构	National Research Foundation of Korea (NRF)National Research Foundation of Korea [NRF-2011-616-F00013] ; BK21Plus program, Ministry of Education, Republic of Korea
作者邮箱	atikgnu@gmail.com ; hyun@gnu.ac.kr
作品OA属性	hybrid
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24982
专题	中科院北方资源植物重点实验室
作者单位	1.Gyeongsang Natl Univ, IALS, Div Appl Life Sci BK21Plus, Jinju 660701, South Korea 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Natl Inst Biotechnol, Dhaka 1349, Bangladesh 4.Korea Forest Res Inst, Div Forest Biotechnol, Suwon 441847, South Korea 5.Gyeongsang Natl Univ, IALS, Dept Appl Biol, Jinju 660701, South Korea
推荐引用方式 GB/T 7714	Atikur, Rahman Md,Yong-goo, Kim,Iftekhar, Alam,et al. Proteome analysis of alfalfa roots in response to water deficit stress[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(6):1275-1285.
APA	Atikur, Rahman Md.,Yong-goo, Kim.,Iftekhar, Alam.,Liu Gong-she.,Hyoshin, Lee.,...&Byung-Hyun, Lee.(2016).Proteome analysis of alfalfa roots in response to water deficit stress.JOURNAL OF INTEGRATIVE AGRICULTURE,15(6),1275-1285.
MLA	Atikur, Rahman Md,et al."Proteome analysis of alfalfa roots in response to water deficit stress".JOURNAL OF INTEGRATIVE AGRICULTURE 15.6(2016):1275-1285.

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