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Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence
Wang, Yangyang1; Ji, Kuixian; Shen, Shihua; Chen, Hui
2016
发表期刊JOURNAL OF PROTEOMICS
ISSN1874-3919
卷号143页码:401-415
摘要A comparison of protein profiles between prolamellar bodies from dark-grown etioplasts and thylakoid membranes from de-etioplasts illuminated respectively for 1, 5 and 9 h revealed 155 differentially expressed CBB-stained spots. Clear results showed that the nonphototransformable pchlide(627-632) was the dominant pigment form in the PLBs of rice etioplasts during plant development in dark and transformed slowly to chlorophyllide in rice etioplasts when exposed to light. The light-induced accumulation of ACC oxidase, which catalyzes the final step of ethylene synthesis using ACC as substrate, would facilitate chlorophyll synthesis by inducing PORa/b expression via ethylene signaling. It could be also suggested that cyclic electron transport might play an important role in generation of ATP for carbon fixation and photoprotection of photosystems from excessive light in prothylakoid. Furthermore, the overproduction of ClpC1, which targets proteins to the ClpPR core complex for degradation, was observed only in Stage 1, during which period PLBs disrupted and converted into prothylakoids, suggesting that ClpC1 was of particular importance for disassembly of PLBs of etioplasts when exposed to light. This study revealed the possible biochemical and physiological processes lead to the formation of functional thylakoid membranes. Biological significance: In this study, we monitored the light-induced transformation of prolamellar bodies into thylakoid membranes, which is correlated to the biogenesis of photosynthetic apparatus involving a complex cascade of biochemical and structural events. Three stages of thylakoid development classified according to the thylakoid development status (Adam et al., 2011) were studied for biogenesis of photosynthetic apparatus: Stage 1, prothylakoids emerge from the disrupted PLB5; Stage 2, prothylakoids converted into primary thylalcoids which were dispersed in the stroma; Stage 3, the continuous grana and stroma thylakoids are formed. The development stage-dependent changes in the proteomic profile of the thylakoids were analyzed by two-dimensional electrophoresis (2-DE). This information was complemented with the steady-state 77 K chlorophyll fluorescence of thylakoids at the corresponding development stage. Together, these analyses allowed us to further understand the molecular processes connected to the formation of functional thylakoid membranes. (C) 2016 Elsevier B.V. All rights reserved.
关键词Thylakoid Development Proteomics Chlorophyll
学科领域Biochemical Research Methods
DOI10.1016/j.jprot.2016.04.040
收录类别SCI
语种英语
WOS关键词CYCLIC ELECTRON-TRANSPORT ; SYNECHOCOCCUS SP PCC7942 ; CHLOROPHYLL-A/B-PROTEIN ; PSBA MESSENGER-RNA ; 3 DNAK PROTEINS ; PHOTOSYSTEM-II ; PROTOCHLOROPHYLLIDE OXIDOREDUCTASE ; RUBISCO ACTIVASE ; ENERGY-TRANSFER ; LARGE SUBUNIT
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000381231400036
出版者ELSEVIER
Special IssueSI
文献子类Article
出版地AMSTERDAM
EISSN1876-7737
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30971716] ; National Program on Key Basic Research Project (973 program)National Basic Research Program of China [2009CB118500]
作者邮箱chenh@ibcas.ac.cn
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/24987
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Wang, Yangyang,Ji, Kuixian,Shen, Shihua,et al. Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence[J]. JOURNAL OF PROTEOMICS,2016,143:401-415.
APA Wang, Yangyang,Ji, Kuixian,Shen, Shihua,&Chen, Hui.(2016).Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence.JOURNAL OF PROTEOMICS,143,401-415.
MLA Wang, Yangyang,et al."Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence".JOURNAL OF PROTEOMICS 143(2016):401-415.
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