Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)

doi:10.1371/journal.pone.0146880

	Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)
	Guo, Yan-Yan ; Huang, Lai-Qiang ; Liu, Zhong-Jian ; Wang, Xiao-Quan
	2016
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	11 期号:1
摘要	Orchidaceae are one of the largest families of flowering plants, with over 27,000 species described and all orchids are listed in CITES. Moreover, the seedlings of orchid species from the same genus are similar. The objective of DNA barcoding is rapid, accurate, and automated species identification, which may be used to identify illegally traded endangered species from vegetative specimens of Paphiopedilum (Venus slipper), a flagship group for plant conservation with high ornamental and commercial values. Here, we selected eight chloroplast barcodes and nrITS to evaluate their suitability in Venus slippers. The results indicate that all tested barcodes had no barcoding gap and the core plant barcodes showed low resolution for the identification of Venus slippers (18.86%). Of the single-locus barcodes, nrITS is the most efficient for the species identification of the genus (52.27%), whereas matK + atpF-atpH is the most efficient multi-locus combination (28.97%). Therefore, we recommend the combination of matK + atpF-atpH + ITS as a barcode for Venus slippers. Furthermore, there is an upper limit of resolution of the candidate barcodes, and only half of the taxa with multiple samples were identified successfully. The low efficiency of these candidate barcodes in Venus slippers may be caused by relatively recent speciation, the upper limit of the barcodes, and/or the sampling density. Although the discriminatory power is relatively low, DNA barcoding may be a promising tool to identify species involved in illegal trade, which has broad applications and is valuable for orchid conservation.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0146880
收录类别	SCI
语种	英语
WOS关键词	LAND PLANTS ; UNIVERSAL BARCODE ; IDENTIFICATION ; L. ; ORCHIDACEAE ; LOCI ; DISCRIMINATE ; EVOLUTION ; TAXONOMY ; REGIONS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000367888100172
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30730010, 31300179] ; Chinese Academy of Sciences (the 100-Talent Project) ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M550712]
作者邮箱	liuzj@sinicaorchid.org ; xiaoq_wang@ibcas.ac.cn
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25072
专题	系统与进化植物学国家重点实验室
作者单位	1.[Guo, Yan-Yan 2.Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen, Peoples R China 3.Orchid Conservat & Res Ctr Shenzhen, Shenzhen, Peoples R China 4.Tsinghua Univ, Grad Sch Shenzhen, Ctr Biotechnol & BioMed, Shenzhen 518057, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Guo, Yan-Yan,Huang, Lai-Qiang,Liu, Zhong-Jian,et al. Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)[J]. PLOS ONE,2016,11(1).
APA	Guo, Yan-Yan,Huang, Lai-Qiang,Liu, Zhong-Jian,&Wang, Xiao-Quan.(2016).Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum).PLOS ONE,11(1).
MLA	Guo, Yan-Yan,et al."Promise and Challenge of DNA Barcoding in Venus Slipper (Paphiopedilum)".PLOS ONE 11.1(2016).

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