Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants

doi:10.1007/s11120-016-0223-4

	Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants
	Fan, Da-Yong ; Fitzpatrick, Duncan 4; Oguchi, Riichi 1,4; Ma, Weimin 2,4; Kou, Jiancun 3,4; Chow, Wah Soon 4
	2016
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	129 期号:3 页码:239-251
摘要	Sixty years ago Arnon and co-workers discovered photophosphorylation driven by a cyclic electron flux (CEF) around Photosystem I. Since then understanding the physiological roles and the regulation of CEF has progressed, mainly via genetic approaches. One basic problem remains, however: quantifying CEF in the absence of a net product. Quantification of CEF under physiological conditions is a crucial prerequisite for investigating the physiological roles of CEF. Here we summarize current progress in methods of CEF quantification in leaves and, in some cases, in isolated thylakoids, of C3 plants. Evidently, all present methods have their own shortcomings. We conclude that to quantify CEF in vivo, the best way currently is to measure the electron flux through PS I (ETR1) and that through PS II and PS I in series (ETR2) for the whole leaf tissue under identical conditions. The difference between ETR1 and ETR2 is an upper estimate of CEF, mainly consisting, in C3 plants, of a major PGR5-PGRL1-dependent CEF component and a minor chloroplast NDH-dependent component, where PGR5 stands for Proton Gradient Regulation 5 protein, PGRL1 for PGR5-like photosynthesis phenotype 1, and NDH for Chloroplast NADH dehydrogenase-like complex. These two CEF components can be separated by the use of antimycin A to inhibit the former (major) component. Membrane inlet mass spectrometry utilizing stable oxygen isotopes provides a reliable estimation of ETR2, whilst ETR1 can be estimated from a method based on the photochemical yield of PS I, Y(I). However, some issues for the recommended method remain unresolved.
关键词	Cyclic electron flux Linear electron flux Membrane inlet mass spectrometry P700 Photosystem I Photosystem II
学科领域	Plant Sciences
DOI	10.1007/s11120-016-0223-4
收录类别	SCI
语种	英语
WOS关键词	WATER-WATER CYCLE ; INDUCED ABSORBENCY CHANGES ; EXCESS EXCITATION-ENERGY ; THYLAKOID MEMBRANES ; QUANTUM YIELD ; CHLAMYDOMONAS-REINHARDTII ; REDOX STATE ; C-3 PLANTS ; 820 NM ; FLOW
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000383584600003
出版者	SPRINGER
Special Issue	SI
文献子类	Review
出版地	DORDRECHT
EISSN	1573-5079
资助机构	Australian Research CouncilAustralian Research Council [DP1093827] ; Knowledge Innovation Program of the Chinese Academy of SciencesChinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-XB3-09-02] ; NNSF of ChinaNational Natural Science Foundation of China (NSFC) [31370424]
作者邮箱	fandayong@ibcas.ac.cn ; duncan.fitzpatrick@anu.edu.au ; riichi.oguchi.a6@tohoku.ac.jp ; wma@shnu.edu.cn ; jiancun02@163.com ; Fred.Chow@anu.edu.au
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25152
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Tohoku Univ, Grad Sch Life Sci, Sendai, Miyagi 9808578, Japan 3.Shanghai Normal Univ, Coll Life & Environm Sci, Guilin Rd 100, Shanghai 200234, Peoples R China 4.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 5.Australian Natl Univ, Res Sch Biol, Div Plant Sci, 46 Sullivans Creek Rd, Acton, ACT 2601, Australia
推荐引用方式 GB/T 7714	Fan, Da-Yong,Fitzpatrick, Duncan,Oguchi, Riichi,et al. Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants[J]. PHOTOSYNTHESIS RESEARCH,2016,129(3):239-251.
APA	Fan, Da-Yong,Fitzpatrick, Duncan,Oguchi, Riichi,Ma, Weimin,Kou, Jiancun,&Chow, Wah Soon.(2016).Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants.PHOTOSYNTHESIS RESEARCH,129(3),239-251.
MLA	Fan, Da-Yong,et al."Obstacles in the quantification of the cyclic electron flux around Photosystem I in leaves of C3 plants".PHOTOSYNTHESIS RESEARCH 129.3(2016):239-251.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Fan-2016-Obstacles i（754KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文