A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice

doi:10.1093/pcp/pcw073

	A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice
	Yang, An ; Zhang, Wen-Hao
	2016
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	57 期号:6 页码:1271-1280
摘要	Plants have evolved two distinct strategies to acquire iron (Fe) from soils. However, the regulatory mechanisms underlying the Fe acquisition remain largely elusive. There is emerging evidence that small GTPases are involved in the responses of plants to environmental cues. Here, we identified a gene encoding a small GTPase, OsRab6a, in rice and characterized its role in Fe acquisition by generating transgenic rice plants with overexpression and knockdown of OsRab6a. OsRab6a shared conserved functional domains with other known members of the Rab subfamily and localized ubiquitously in the cytoplasm and nucleus. The expression of OsRab6a was rapidly and transiently up-regulated by Fe deficiency. No differences in growth and development among the OsRab6a-overexpression, OsRab6a-RNAi (RNA interference) and wild-type plants were detected when grown in Fe-sufficient medium. However, over-expression of OsRab6a in rice plants conferred greater tolerance to Fe deficiency than RNAi and wild-type plants, as evidenced by higher seedling height, and greater biomass, Chl contents and Fe concentrations in shoots, roots and grains in the overexpression lines than wild-type and RNAi plants. Moreover, the overexpression lines exhibited larger root systems than wild-type and RNAi plants in Fe-deficient medium. Exposure to Fe-deficient medium led to up-regulation of OsIRO2, OsIRT1, OsNAS1 and OsNAS2 in both wild-type and transgenic rice plants, with themagnitude of up-regulation positively correlated with the expression levels of OsRab6a. These results may suggest that OsRab6a plays an important role in the regulation of Fe acquisition in rice plants by modulating physiological processes involved in Fe acquisition and root system architecture in response to Fe-deficient medium.
关键词	Fe acquisition Fe deficiency OsRab6a Rice (Oryza sativa) Root system Small GTPase
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcw073
收录类别	SCI
语种	英语
WOS关键词	BINDING PROTEIN GENE ; FE-DEFICIENCY STRESS ; TRANSCRIPTION FACTOR ; EXPRESSION ; RESPONSES ; OVEREXPRESSION ; TRANSPORT ; VESICLES ; PLANTS ; SALT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000379752100014
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31301832] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010401]
作者邮箱	whzhang@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25181
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, An,Zhang, Wen-Hao. A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice[J]. PLANT AND CELL PHYSIOLOGY,2016,57(6):1271-1280.
APA	Yang, An,&Zhang, Wen-Hao.(2016).A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice.PLANT AND CELL PHYSIOLOGY,57(6),1271-1280.
MLA	Yang, An,et al."A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice".PLANT AND CELL PHYSIOLOGY 57.6(2016):1271-1280.

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