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The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species | |
Qi, Shilian1,2; Lin, Qingfang1,2; Zhu, Huishan1; Gao, Fenghua1; Zhang, Wenhao3; Hua, Xuejun1 | |
2016 | |
发表期刊 | PLANT AND CELL PHYSIOLOGY |
ISSN | 0032-0781 |
卷号 | 57期号:3页码:528-539 |
摘要 | Protein ubiquitination in plants plays critical roles in many biological processes, including adaptation to abiotic stresses. Previously, RING finger E3 ligase has been characterized during salt stress response in several plant species, but little is known about its function in tomato. Here, we report that SpRing, a stress-inducible gene, is involved in salt stress signaling in wild tomato species Solanum pimpinellifolium 'PI365967'. In vitro ubiquitination assay revealed that SpRing is an E3 ubiquitin ligase and the RING finger conserved region is required for its activity. SpRing is expressed in all tissues of wild tomato and up-regulated by salt, drought and osmotic stresses, but repressed by low temperature. Green fluorescent protein (GFP) fusion analysis showed that SpRing is localized at the endoplasmic reticulum. Silencing of SpRing through a virus-induced gene silencing approach led to increased sensitivity to salt stress in wild tomato. Overexpression of SpRing in Arabidopsis thaliana resulted in enhanced salt tolerance during seed germination and early seedling development. The expression levels of certain key stress-related genes are altered both in SpRing-overexpressing Arabidopsis plants and virus-induced gene silenced tomato seedlings. Taken together, our results indicate that SpRing is involved in salt stress and functions as a positive regulator of salt tolerance. |
关键词 | E3 Ligase RING-H2 salt tolerance Solanum pimpinellifolium VIGS |
学科领域 | Plant Sciences ; Cell Biology |
DOI | 10.1093/pcp/pcw006 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | NA+/H+ ANTIPORTER SOS1 ; TOBACCO-MOSAIC-VIRUS ; ARABIDOPSIS-THALIANA ; CHLOROPHYLL FLUORESCENCE ; HYPERSENSITIVE RESPONSE ; LYCOPERSICON-PENNELLII ; ANTIOXIDATIVE SYSTEM ; TRANSGENIC ALFALFA ; DROUGHT RESPONSE ; UBIQUITIN |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000374572400008 |
出版者 | OXFORD UNIV PRESS |
文献子类 | Article |
出版地 | OXFORD |
EISSN | 1471-9053 |
资助机构 | National Project for Transgenic Research [2015ZX08009-003] |
作者邮箱 | xjhua@ibcas.ac.cn |
作品OA属性 | Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25198 |
专题 | 植被与环境变化国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Qi, Shilian,Lin, Qingfang,Zhu, Huishan,et al. The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species[J]. PLANT AND CELL PHYSIOLOGY,2016,57(3):528-539. |
APA | Qi, Shilian,Lin, Qingfang,Zhu, Huishan,Gao, Fenghua,Zhang, Wenhao,&Hua, Xuejun.(2016).The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species.PLANT AND CELL PHYSIOLOGY,57(3),528-539. |
MLA | Qi, Shilian,et al."The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species".PLANT AND CELL PHYSIOLOGY 57.3(2016):528-539. |
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Qi-2016-The RING Fin(1674KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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