IB-CAS  > 植被与环境变化国家重点实验室
The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species
Qi, Shilian1,2; Lin, Qingfang1,2; Zhu, Huishan1; Gao, Fenghua1; Zhang, Wenhao3; Hua, Xuejun1
2016
发表期刊PLANT AND CELL PHYSIOLOGY
ISSN0032-0781
卷号57期号:3页码:528-539
摘要Protein ubiquitination in plants plays critical roles in many biological processes, including adaptation to abiotic stresses. Previously, RING finger E3 ligase has been characterized during salt stress response in several plant species, but little is known about its function in tomato. Here, we report that SpRing, a stress-inducible gene, is involved in salt stress signaling in wild tomato species Solanum pimpinellifolium 'PI365967'. In vitro ubiquitination assay revealed that SpRing is an E3 ubiquitin ligase and the RING finger conserved region is required for its activity. SpRing is expressed in all tissues of wild tomato and up-regulated by salt, drought and osmotic stresses, but repressed by low temperature. Green fluorescent protein (GFP) fusion analysis showed that SpRing is localized at the endoplasmic reticulum. Silencing of SpRing through a virus-induced gene silencing approach led to increased sensitivity to salt stress in wild tomato. Overexpression of SpRing in Arabidopsis thaliana resulted in enhanced salt tolerance during seed germination and early seedling development. The expression levels of certain key stress-related genes are altered both in SpRing-overexpressing Arabidopsis plants and virus-induced gene silenced tomato seedlings. Taken together, our results indicate that SpRing is involved in salt stress and functions as a positive regulator of salt tolerance.
关键词E3 Ligase RING-H2 salt tolerance Solanum pimpinellifolium VIGS
学科领域Plant Sciences ; Cell Biology
DOI10.1093/pcp/pcw006
收录类别SCI
语种英语
WOS关键词NA+/H+ ANTIPORTER SOS1 ; TOBACCO-MOSAIC-VIRUS ; ARABIDOPSIS-THALIANA ; CHLOROPHYLL FLUORESCENCE ; HYPERSENSITIVE RESPONSE ; LYCOPERSICON-PENNELLII ; ANTIOXIDATIVE SYSTEM ; TRANSGENIC ALFALFA ; DROUGHT RESPONSE ; UBIQUITIN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000374572400008
出版者OXFORD UNIV PRESS
文献子类Article
出版地OXFORD
EISSN1471-9053
资助机构National Project for Transgenic Research [2015ZX08009-003]
作者邮箱xjhua@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/25198
专题植被与环境变化国家重点实验室
作者单位1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Qi, Shilian,Lin, Qingfang,Zhu, Huishan,et al. The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species[J]. PLANT AND CELL PHYSIOLOGY,2016,57(3):528-539.
APA Qi, Shilian,Lin, Qingfang,Zhu, Huishan,Gao, Fenghua,Zhang, Wenhao,&Hua, Xuejun.(2016).The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species.PLANT AND CELL PHYSIOLOGY,57(3),528-539.
MLA Qi, Shilian,et al."The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species".PLANT AND CELL PHYSIOLOGY 57.3(2016):528-539.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Qi-2016-The RING Fin(1674KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qi, Shilian]的文章
[Lin, Qingfang]的文章
[Zhu, Huishan]的文章
百度学术
百度学术中相似的文章
[Qi, Shilian]的文章
[Lin, Qingfang]的文章
[Zhu, Huishan]的文章
必应学术
必应学术中相似的文章
[Qi, Shilian]的文章
[Lin, Qingfang]的文章
[Zhu, Huishan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Qi-2016-The RING Finger E3 Ligase SpRing is a.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。