OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice

doi:10.1093/jxb/erv515

	OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice
	Dai, Xiaoyan ; Wang, Yuanyuan ; Zhang, Wen-Hao
	2016
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	67 期号:3 页码:947-960
摘要	The WRKY transcription factor family has 109 members in the rice genome, and has been reported to be involved in the regulation of biotic and abiotic stress in plants. Here, we demonstrated that a rice OsWRKY74 belonging to group III of the WRKY transcription factor family was involved in tolerance to phosphate (Pi) starvation. OsWRKY74 was localized in the nucleus and mainly expressed in roots and leaves. Overexpression of OsWRKY74 significantly enhanced tolerance to Pi starvation, whereas transgenic lines with down-regulation of OsWRKY74 were sensitive to Pi starvation. Root and shoot biomass, and phosphorus (P) concentration in rice OsWRKY74-overexpressing plants were similar to 16% higher than those of wild-type (WT) plants in Pi-deficient hydroponic solution. In soil pot experiments, >24% increases in tiller number, grain weight and P concentration were observed in rice OsWRKY74-overexpressing plants compared to WT plants when grown in P-deficient medium. Furthermore, Pi starvation-induced changes in root system architecture were more profound in OsWRKY74-overexpressing plants than in WT plants. Expression patterns of a number of Pi-responsive genes were altered in the OsWRKY74-overexpressing and RNA interference lines. In addition, OsWRKY74 may also be involved in the response to deficiencies in iron (Fe) and nitrogen (N) as well as cold stress in rice. In Pi-deficient conditions, OsWRKY74-overexpressing plants exhibited greater accumulation of Fe and up-regulation of the cold-responsive genes than WT plants. These findings highlight the role of OsWRKY74 in modulation of Pi homeostasis and potential crosstalk between P starvation and Fe starvation, and cold stress in rice.
关键词	OsWRKY74 phosphate starvation rice (Oryza sativa) root system architecture transgenic
学科领域	Plant Sciences
DOI	10.1093/jxb/erv515
收录类别	SCI
语种	英语
WOS关键词	ROOT-SYSTEM ARCHITECTURE ; PHOSPHORUS ACQUISITION ; MEDICAGO-TRUNCATULA ; GENE-EXPRESSION ; COLD TOLERANCE ; ARABIDOPSIS ; RESPONSES ; HOMEOSTASIS ; DEFICIENCY ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000371019800033
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170243, 31570266, 30870188]
作者邮箱	whzhang@ibcas.ac.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：176[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25203
专题	植被与环境变化国家重点实验室
作者单位	1.[Dai, Xiaoyan 2.Wang, Yuanyuan 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Dai, Xiaoyan,Wang, Yuanyuan,Zhang, Wen-Hao. OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(3):947-960.
APA	Dai, Xiaoyan,Wang, Yuanyuan,&Zhang, Wen-Hao.(2016).OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice.JOURNAL OF EXPERIMENTAL BOTANY,67(3),947-960.
MLA	Dai, Xiaoyan,et al."OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice".JOURNAL OF EXPERIMENTAL BOTANY 67.3(2016):947-960.

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