Molecular basis of 1-methylcyclopropene regulating organic acid metabolism in apple fruit during storage

doi:10.1016/j.postharvbio.2016.02.001

	Molecular basis of 1-methylcyclopropene regulating organic acid metabolism in apple fruit during storage
	Liu, Ruiling 1; Wang, Yuying ; Qin, Guozheng; Tian, Shiping1
	2016
发表期刊	POSTHARVEST BIOLOGY AND TECHNOLOGY
ISSN	0925-5214
卷号	117 页码:57-63
摘要	Organic acids play a critical role in flavor and overall organoleptic quality of fruit. Malate has been considered to be the major organic acid determining apple fruit acidity. Here, we found that 1-methylcyclopropene (1-MCP) delayed the loss of malate and citrate during storage. 1-MCP-treated fruit showed higher level of phosphoenol pyruvate carboxylase (PEPC) and cytosolic NAD-dependent malate dehydrogenase (cyNAD-MDH) activities, but lower or similar phosphoenolpyruvate carboxylase kinase (PEPCK) and cytosolic NAD-dependent malate dehydrogenase (cyNADP-ME) activities compared to the control. Moreover, the expression of acid transport genes, including MdVHA-A, MdVHP and Ma1, was up-regulated in 1-MCP-treated fruit, resulting in retention of malate in the vacuole, and eventual higher malate content. Hence, 1-MCP maintained fruit acidity by regulating the balance between malate biosynthesis and degradation. These findings provide molecular evidence for understanding the mode of action of 1-MCP regulating organic acid metabolism in apple fruit during storage. (C) 2016 Elsevier B.V. All rights reserved.
关键词	1-MCP Organic acid metabolism Molecular basis Apple fruit Postharvest storage
学科领域	Agronomy ; Food Science & Technology ; Horticulture
DOI	10.1016/j.postharvbio.2016.02.001
收录类别	SCI
语种	英语
WOS关键词	DEVELOPMENTAL-CHANGES ; COMPOSITIONAL CHANGES ; MALATE METABOLISM ; STRESS TOLERANCE ; H+-PPASE ; ACCUMULATION ; EXPRESSION ; ETHYLENE ; GENE ; PHOTOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373717600007
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-2356
资助机构	National Basic Research Program of China (973 Program)National Basic Research Program of China [2011CB100604] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31030051]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25238
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ruiling,Wang, Yuying,Qin, Guozheng,et al. Molecular basis of 1-methylcyclopropene regulating organic acid metabolism in apple fruit during storage[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2016,117:57-63.
APA	Liu, Ruiling,Wang, Yuying,Qin, Guozheng,&Tian, Shiping.(2016).Molecular basis of 1-methylcyclopropene regulating organic acid metabolism in apple fruit during storage.POSTHARVEST BIOLOGY AND TECHNOLOGY,117,57-63.
MLA	Liu, Ruiling,et al."Molecular basis of 1-methylcyclopropene regulating organic acid metabolism in apple fruit during storage".POSTHARVEST BIOLOGY AND TECHNOLOGY 117(2016):57-63.

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