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| New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis) | |
Zhao, Pincang1; Liu, Panpan1; Yuan, Guangxiao1; Jia, Junting1; Li, Xiaoxia; Qi, Dongmei; Chen, Shuangyan; Ma, Tian; Liu, Gongshe ; Cheng, Liqin
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| 2016 | |
| 发表期刊 | FRONTIERS IN PLANT SCIENCE
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| ISSN | 1664-462X |
| 卷号 | 7 |
| 摘要 | Water is a critical environmental factor that restricts the geographic distribution of plants. Sheepgrass [Leyrnus chinensis, (Trin.) Tzvel] is an important forage grass in the Eurasia Steppe and a close germplasm for wheat and barley. This native grass adapts well to adverse environments such as cold, salinity, alkalinity and drought, and it can survive when the soil moisture may be less than 6% in dry seasons. However, little is known about how sheepgrass tolerates water stress at the molecular level. Here, drought stress experiment and RNA-sequencing (RNA-seq) was performed in three pools of RNA samples (control, drought stress, and rewatering). We found that sheepgrass seedlings could still survive when the soil water content (SWC) was reduced to 14.09%. Differentially expressed genes (DEGs) analysis showed that 7320 genes exhibited significant responses to drought stress. Of these DEGs, 2671 presented opposite expression trends before and after rewatering. Furthermore, 680 putative sheepgrass-specific water responsive genes were revealed that can be studied deeply. Gene ontology (GO) annotation revealed that stress-associated genes were activated extensively by drought treatment. Interestingly, cold stress-related genes were up-regulated greatly after drought stress. The DEGs of MAPK and calcium signal pathways, plant hormone ABA, jasmonate, ethylene, brassinosteroid signal pathways, cold response CBF pathway participated coordinatively in sheepgrass drought stress response. In addition, we identified 288 putative transcription factors (TFs) involved in drought response, among them, the WRKY, NAG, AP2/ERF, bHLH, bZIP, and MYB families were enriched, and might play crucial and significant roles in drought stress response of sheepgrass. Our research provided new and valuable information for understanding the mechanism of drought tolerance in sheepgrass. Moreover, the identification of genes involved in drought response can facilitate the genetic improvement of crops by molecular breeding. |
| 关键词 | sheepgrass drought stress RNA-seq ABA-dependent pathway transcription factors |
| 学科领域 | Plant Sciences |
| DOI | 10.3389/fpls.2016.00954 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | TRANSCRIPTOME-WIDE IDENTIFICATION ; OXYGEN SPECIES PRODUCTION ; SALT-INDUCED GENE ; ABSCISIC-ACID ; RNA-SEQ ; ARABIDOPSIS-THALIANA ; METHYL JASMONATE ; TREHALOSE BIOSYNTHESIS ; TRANSGENIC ARABIDOPSIS ; SIGNAL-TRANSDUCTION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000378849500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 文献子类 | Article |
| 出版地 | LAUSANNE |
| 资助机构 | National Basic Research Program of China (973 program)National Basic Research Program of China [2014CB138704] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences (STS) [KFJ-EW-STS-119] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170316] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2014ZX08009-003-002] |
| 作者邮箱 | liugs@ibcas.ac.cn ; lqcheng@ibcas.ac.cn |
| 作品OA属性 | gold, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25239 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Pincang,Liu, Panpan,Yuan, Guangxiao,et al. New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)[J]. FRONTIERS IN PLANT SCIENCE,2016,7. |
| APA | Zhao, Pincang.,Liu, Panpan.,Yuan, Guangxiao.,Jia, Junting.,Li, Xiaoxia.,...&Cheng, Liqin.(2016).New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis).FRONTIERS IN PLANT SCIENCE,7. |
| MLA | Zhao, Pincang,et al."New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)".FRONTIERS IN PLANT SCIENCE 7(2016). |
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| S1664462X_S1664462X1（8932KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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