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Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds | |
Zhang, Ying-Xue; Xu, Heng-Heng; Liu, Shu-Jun; Li, Ni1; Wang, Wei-Qing; Moller, Ian M.; Song, Song-Quan | |
2016 | |
发表期刊 | FRONTIERS IN PLANT SCIENCE |
ISSN | 1664-462X |
卷号 | 7 |
摘要 | Seed aging is a process that results in a delayed germination, a decreased germination percentage, and finally a total loss of seed viability. However, the mechanism of seed aging is poorly understood. In the present study, Yliangyou 2 hybrid rice (Oryza sativa L.) seeds were artificially aged at 100% relative humidity and 40 degrees C, and the effect of artificial aging on germination, germination time course and the change in protein profiles of embryo and endosperm was studied to understand the molecular mechanism behind seed aging. With an increasing duration of artificial aging, the germination percentage and germination rate of hybrid rice seeds decreased. By comparing the protein profiles from the seeds aged for 0, 10 and 25 days, a total of 91 and 100 protein spots were found to show a significant change of more than 2-fold (P < 0.05) in abundance, and 71 and 79 protein spots were identified, in embryos and endosperms, respectively. The great majority of these proteins increased in abundance in embryos (95%) and decreased in abundance in endosperms (99%). In embryos, most of the identified proteins were associated with energy (30%), with cell defense and rescue (28%), and with storage protein (18%). In endosperms, most of the identified proteins were involved in metabolism (37%), in energy (27%), and in protein synthesis and destination (11%). The most marked change was the increased abundance of many glycolytic enzymes together with the two fermentation enzymes pyruvate decarboxylase and alcohol dehydrogenase in the embryos during aging. We hypothesize that the decreased viability of hybrid rice seeds during artificial aging is caused by the development of hypoxic conditions in the embryos followed by ethanol accumulation. |
关键词 | Yliangyou 2 hybrid rice proteome embryo endosperm seed aging |
学科领域 | Plant Sciences |
DOI | 10.3389/fpls.2016.01394 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | WIDE CHARACTERIZATION ; ARABIDOPSIS-THALIANA ; SUGAR-BEET ; SATIVA L. ; VIGOR ; DETERIORATION ; GERMINATION ; BIOSYNTHESIS ; EXPRESSION ; MUTANTS |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000383532100001 |
出版者 | FRONTIERS MEDIA SA |
文献子类 | Article |
出版地 | LAUSANNE |
资助机构 | National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of SciencesChinese Academy of Sciences |
作者邮箱 | sqsong@ibcas.ac.cn |
作品OA属性 | Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25245 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China 3.Moller, Ian M.] Aarhus Univ, Dept Mol Biol & Genet, Flakkebjerg, Denmark |
推荐引用方式 GB/T 7714 | Zhang, Ying-Xue,Xu, Heng-Heng,Liu, Shu-Jun,et al. Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds[J]. FRONTIERS IN PLANT SCIENCE,2016,7. |
APA | Zhang, Ying-Xue.,Xu, Heng-Heng.,Liu, Shu-Jun.,Li, Ni.,Wang, Wei-Qing.,...&Song, Song-Quan.(2016).Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds.FRONTIERS IN PLANT SCIENCE,7. |
MLA | Zhang, Ying-Xue,et al."Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds".FRONTIERS IN PLANT SCIENCE 7(2016). |
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S1664462X_S1664462X1(1990KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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