Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds

doi:10.1016/j.jplph.2016.02.021

	Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds
	Liu, Shu-Jun ; Xu, Heng-Heng ; Wang, Wei-Qing ; Li, Ni ; Wang, Wei-Ping ; Lu, Zhuang ; Moller, Ian Max 3; Song, Song-Quan
	2016
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	196-197 页码:79-92
摘要	Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 mu M inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 mu M completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development. (C) 2016 Elsevier GmbH. All rights reserved.
关键词	Embryo Oryza sativa Proteome analysis Seed germination Seedling establishment
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2016.02.021
收录类别	SCI
语种	英语
WOS关键词	PROTEOMIC ANALYSIS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; MESSENGER-RNAS ; LIPOXYGENASE ; MOBILIZATION ; GROWTH ; ROLES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000376162400009
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of Sciences Visiting Professorship for senior international scientists
作者邮箱	sqsong@ibcas.ac.cn
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25246
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Hunan Hybrid Rice Res Center, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark
推荐引用方式 GB/T 7714	Liu, Shu-Jun,Xu, Heng-Heng,Wang, Wei-Qing,et al. Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds[J]. JOURNAL OF PLANT PHYSIOLOGY,2016,196-197:79-92.
APA	Liu, Shu-Jun.,Xu, Heng-Heng.,Wang, Wei-Qing.,Li, Ni.,Wang, Wei-Ping.,...&Song, Song-Quan.(2016).Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds.JOURNAL OF PLANT PHYSIOLOGY,196-197,79-92.
MLA	Liu, Shu-Jun,et al."Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds".JOURNAL OF PLANT PHYSIOLOGY 196-197(2016):79-92.

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