Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds

doi:10.1104/pp.16.00384

	Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds
	Cao, Dechang 2; Xu, Huimin ; Zhao, Yuanyuan ; Deng, Xin1 ; Liu, Yongxiu1 ; Soppe, Wim J. J.; Lin, Jinxing 1
	2016
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	172 期号:4 页码:2347-2362
摘要	Seeds with physiological dormancy usually experience primary and secondary dormancy in the nature; however, little is known about the differential regulation of primary and secondary dormancy. We combined multiple approaches to investigate cytological changes, hormonal levels, and gene expression dynamics in Cunninghamia lanceolata seeds during primary dormancy release and secondary dormancy induction. Light microscopy and transmission electron microscopy revealed that protein bodies in the embryo cells coalesced during primary dormancy release and then separated during secondary dormancy induction. Transcriptomic profiling demonstrated that expression of genes negatively regulating gibberellic acid (GA) sensitivity reduced specifically during primary dormancy release, whereas the expression of genes positively regulating abscisic acid (ABA) biosynthesis increased during secondary dormancy induction. Parallel analysis of RNA ends revealed uncapped transcripts for; 55% of all unigenes. A negative correlation between fold changes in expression levels of uncapped versus capped mRNAs was observed during primary dormancy release. However, this correlation was loose during secondary dormancy induction. Our analyses suggest that the reversible changes in cytology and gene expression during dormancy release and induction are related to ABA/GA balance. Moreover, mRNA degradation functions as a critical posttranscriptional regulator during primary dormancy release. These findings provide a mechanistic framework for understanding physiological dormancy in seeds.
学科领域	Plant Sciences
DOI	10.1104/pp.16.00384
收录类别	SCI
语种	英语
WOS关键词	UNCAPPED MESSENGER-RNAS ; GENE-EXPRESSION ; ABSCISIC-ACID ; PARALLEL ANALYSIS ; ARABIDOPSIS CVI ; MIRNA TARGETS ; CHINESE FIR ; IDENTIFICATION ; GERMINATION ; BIOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000391173400022
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370212, 31270224] ; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design ; Programme of Introducing Talents of Discipline to Universities (111 Project)Ministry of Education, China - 111 Project [B13007] ; Programme for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13047] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2013M530536, 2015M570943]
作者邮箱	linjinxing@bjfu.edu.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25248
专题	中科院北方资源植物重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Max Planck Inst Chem Ecol, Dept Mol Ecol, D-07745 Jena, Germany 4.Soppe, Wim J. J.] Max Planck Inst Plant Breeding Res, Dept Plant Breeding & Genet, D-50829 Cologne, Germany
推荐引用方式 GB/T 7714	Cao, Dechang,Xu, Huimin,Zhao, Yuanyuan,et al. Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds[J]. PLANT PHYSIOLOGY,2016,172(4):2347-2362.
APA	Cao, Dechang.,Xu, Huimin.,Zhao, Yuanyuan.,Deng, Xin.,Liu, Yongxiu.,...&Lin, Jinxing.(2016).Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds.PLANT PHYSIOLOGY,172(4),2347-2362.
MLA	Cao, Dechang,et al."Transcriptome and Degradome Sequencing Reveals Dormancy Mechanisms of Cunninghamia lanceolata Seeds".PLANT PHYSIOLOGY 172.4(2016):2347-2362.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Cao-2016-Transcripto（2684KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文