A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening

doi:10.1104/pp.16.01269

	A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening
	Qin, Guozheng; Zhu, Zhu ; Wang, Weihao 1; Cai, Jianghua 1; Chen, Yong 1; Li, Li 2; Tian, Shiping1
	2016
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	172 期号:3 页码:1596-1611
摘要	Fruit ripening is a complex process that involves a series of physiological and biochemical changes that ultimately influence fruit quality traits, such as color and flavor. Sugar metabolism is an important factor in ripening, and there is evidence that it influences various aspects of ripening, although the associated mechanism is not well understood. In this study, we identified and analyzed the expression of 36 genes involved in Suc metabolism in ripening tomato (Solanum lycopersicum) fruit. Chromatin immunoprecipitation and gel mobility shift assays indicated that SlVIF, which encodes a vacuolar invertase inhibitor, and SlVI, encoding a vacuolar invertase, are directly regulated by the global fruit ripening regulator RIPENING INHIBITOR (RIN). Moreover, we showed that SlVIF physically interacts with SlVI to control Suc metabolism. Repression of SlVIF by RNA interference delayed tomato fruit ripening, while overexpression of SlVIF accelerated ripening, with concomitant changes in lycopene production and ethylene biosynthesis. An isobaric tags for relative and absolute quantification-based quantitative proteomic analysis further indicated that the abundance of a set of proteins involved in fruit ripening was altered by suppressing SlVIF expression, including proteins associated with lycopene generation and ethylene synthesis. These findings provide evidence for the role of Suc in promoting fruit ripening and establish that SlVIF contributes to fruit quality and the RIN-mediated ripening regulatory mechanisms, which are of significant agricultural value.
学科领域	Plant Sciences
DOI	10.1104/pp.16.01269
收录类别	SCI
语种	英语
WOS关键词	PLANT DEVELOPMENT ; EXPRESSION ANALYSIS ; SUGAR COMPOSITION ; GENE-EXPRESSION ; MESSENGER-RNA ; POTATO-TUBERS ; ACID ; CLONING ; PROTEIN ; FRUCTOKINASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000391172300019
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2011CB100604] ; Youth Innovation Promotion Association CAS
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：119[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25253
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.USDA, Robert W Holley Ctr Agr Hlth, Agr Res Serv, Ithaca, NY 14853 USA
推荐引用方式 GB/T 7714	Qin, Guozheng,Zhu, Zhu,Wang, Weihao,et al. A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening[J]. PLANT PHYSIOLOGY,2016,172(3):1596-1611.
APA	Qin, Guozheng.,Zhu, Zhu.,Wang, Weihao.,Cai, Jianghua.,Chen, Yong.,...&Tian, Shiping.(2016).A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening.PLANT PHYSIOLOGY,172(3),1596-1611.
MLA	Qin, Guozheng,et al."A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening".PLANT PHYSIOLOGY 172.3(2016):1596-1611.

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