Knowledge Management System Of Institute Of Botany,CAS
| Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy | |
| Li, Xiaojuan; Xing, Jingjing1,2; Qiu, Zongbo3; He, Qihua4; Lin, Jinxing | |
| 2016 | |
| 发表期刊 | MOLECULAR PLANT
 |
| ISSN | 1674-2052 |
| 卷号 | 9期号:9页码:1229-1239 |
| 摘要 | Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluorescence correlation spectroscopy (FCS) is a powerful technique to describe events that occur at the single-molecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dynamics and interactions of membrane proteins. We also discuss the potential applications of this technique in plant biology. |
| 关键词 | fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.molp.2016.06.017 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | CROSS-CORRELATION SPECTROSCOPY ; PLASMA-MEMBRANE ; LIPID RAFTS ; ANOMALOUS DIFFUSION ; LIVING CELLS ; REVEALS ; MICRODOMAINS ; RECEPTOR ; CLATHRIN ; FCS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000386585500001 |
| 出版者 | CELL PRESS |
| 文献子类 | Review |
| 出版地 | CAMBRIDGE |
| EISSN | 1752-9867 |
| 资助机构 | State Key Program of National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31530084] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370212, 31270224] ; Program of Introducing Talents of Discipline to UniversitiesMinistry of Education, China - 111 Project [13007] ; Program for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13047] |
| 作者邮箱 | linjx@ibcas.ac.cn |
| 作品OA属性 | Bronze |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25257 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 4.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 5.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,et al. Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy[J]. MOLECULAR PLANT,2016,9(9):1229-1239. |
| APA | Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,He, Qihua,&Lin, Jinxing.(2016).Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy.MOLECULAR PLANT,9(9),1229-1239. |
| MLA | Li, Xiaojuan,et al."Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy".MOLECULAR PLANT 9.9(2016):1229-1239. |
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| 1-s2.0-S167420521630（2103KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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