Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis

doi:10.1093/pcp/pcw093

	Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis
	Li, Jingrui 1; Wei, Xuezhi 1; Yu, Pengli ; Deng, Xin2,3 ; Xu, Wenxiu ; Ma, Mi ; Zhang, Haiyan
	2016
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	57 期号:8 页码:1720-1731
摘要	Cadmium (Cd) is a transition metal that is highly toxic in biological systems. Anthropogenic emissions of Cd have increased biogeochemical cycling and the amount of Cd in the biosphere. Here we studied the utility of a bacterial Cd-binding protein, CadR, for the remediation of Cd contamination. CadR was successfully targeted to chloroplasts using a constitutive Cauliflower mosaic virus (CaMV) 35S promoter or a shoot-specific Chl a/b-binding protein 2 gene (CAB2) promoter and an RbcS (small subunit of the Rubisco complex) transit peptide. Under short-term (2 d) exposure to Cd, the cadR transgenic plants showed up to a 2.9-fold Cd accumulation in roots compared with untransformed plants. Under medium term (7 d) exposure to Cd, the concentrations of Cd in leaves began to increase but there were no differences between the wild type and the cadR transgenic plants. Under long-term (16 d) exposure to Cd, the cadR transgenic plants accumulated greater amounts of Cd in leaves than the untransformed plants. Total Cd accumulation (mg per plant) in shoots and roots of the plants expressing cadR were significantly higher (up to 3.5-fold in shoots and 5.2-fold in roots) than those of the untransformed plants. We also found that targeting CadR to chloroplasts facilitated chloroplastic metal homeostasis and Chl b accumulation. Our results demonstrate that manipulating chelating capacity in chloroplasts or in the cytoplasm may be effective in modifying both the accumulation of and resistance to Cd.
关键词	Arabidopsis Cadmium (Cd) CadR Chloroplast targeting Mn Fe
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcw093
收录类别	SCI
语种	英语
WOS关键词	METAL HYPERACCUMULATION ; PSEUDOMONAS-AERUGINOSA ; CADMIUM RESISTANCE ; OXIDATIVE STRESS ; TRANSIT PEPTIDE ; SMALL-SUBUNIT ; IRON ; TRANSPORTER ; ROOT ; CHLOROPLASTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000384717100010
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270313, 31470292]
作者邮箱	hyz@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25260
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Shanxi Normal Univ, Coll Life Sci, Linfen 041004, Shanxi, Peoples R China 3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 4.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Li, Jingrui,Wei, Xuezhi,Yu, Pengli,et al. Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2016,57(8):1720-1731.
APA	Li, Jingrui.,Wei, Xuezhi.,Yu, Pengli.,Deng, Xin.,Xu, Wenxiu.,...&Zhang, Haiyan.(2016).Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis.PLANT AND CELL PHYSIOLOGY,57(8),1720-1731.
MLA	Li, Jingrui,et al."Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis".PLANT AND CELL PHYSIOLOGY 57.8(2016):1720-1731.

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