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Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis
Li, Jingrui1; Wei, Xuezhi1; Yu, Pengli; Deng, Xin2,3; Xu, Wenxiu; Ma, Mi; Zhang, Haiyan
2016
发表期刊PLANT AND CELL PHYSIOLOGY
ISSN0032-0781
卷号57期号:8页码:1720-1731
摘要Cadmium (Cd) is a transition metal that is highly toxic in biological systems. Anthropogenic emissions of Cd have increased biogeochemical cycling and the amount of Cd in the biosphere. Here we studied the utility of a bacterial Cd-binding protein, CadR, for the remediation of Cd contamination. CadR was successfully targeted to chloroplasts using a constitutive Cauliflower mosaic virus (CaMV) 35S promoter or a shoot-specific Chl a/b-binding protein 2 gene (CAB2) promoter and an RbcS (small subunit of the Rubisco complex) transit peptide. Under short-term (2 d) exposure to Cd, the cadR transgenic plants showed up to a 2.9-fold Cd accumulation in roots compared with untransformed plants. Under medium term (7 d) exposure to Cd, the concentrations of Cd in leaves began to increase but there were no differences between the wild type and the cadR transgenic plants. Under long-term (16 d) exposure to Cd, the cadR transgenic plants accumulated greater amounts of Cd in leaves than the untransformed plants. Total Cd accumulation (mg per plant) in shoots and roots of the plants expressing cadR were significantly higher (up to 3.5-fold in shoots and 5.2-fold in roots) than those of the untransformed plants. We also found that targeting CadR to chloroplasts facilitated chloroplastic metal homeostasis and Chl b accumulation. Our results demonstrate that manipulating chelating capacity in chloroplasts or in the cytoplasm may be effective in modifying both the accumulation of and resistance to Cd.
关键词Arabidopsis Cadmium (Cd) CadR Chloroplast targeting Mn Fe
学科领域Plant Sciences ; Cell Biology
DOI10.1093/pcp/pcw093
收录类别SCI
语种英语
WOS关键词METAL HYPERACCUMULATION ; PSEUDOMONAS-AERUGINOSA ; CADMIUM RESISTANCE ; OXIDATIVE STRESS ; TRANSIT PEPTIDE ; SMALL-SUBUNIT ; IRON ; TRANSPORTER ; ROOT ; CHLOROPLASTS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000384717100010
出版者OXFORD UNIV PRESS
文献子类Article
出版地OXFORD
EISSN1471-9053
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270313, 31470292]
作者邮箱hyz@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/25260
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Shanxi Normal Univ, Coll Life Sci, Linfen 041004, Shanxi, Peoples R China
3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA
4.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
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Li, Jingrui,Wei, Xuezhi,Yu, Pengli,et al. Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2016,57(8):1720-1731.
APA Li, Jingrui.,Wei, Xuezhi.,Yu, Pengli.,Deng, Xin.,Xu, Wenxiu.,...&Zhang, Haiyan.(2016).Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis.PLANT AND CELL PHYSIOLOGY,57(8),1720-1731.
MLA Li, Jingrui,et al."Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis".PLANT AND CELL PHYSIOLOGY 57.8(2016):1720-1731.
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