Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy

doi:10.3389/fmicb.2016.00601

	Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy
	Li, Boqiang ; Peng, Huaimin 1; Tian, Shiping
	2016
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	7
摘要	Rhodotorula glutinis as an antagonism show good biocontrol performance against various post-harvest diseases in fruits. In the present study, strong attachment capability of R. glutinis to spores and hyphae of Botrytis cinerea was observed. Further analysis showed that certain protein components on the yeast cell surface played critical role during the interaction between R. glutinis and B. cinerea. The components mainly distributed at the poles of yeast cells and might contain glycosylation modification, as tunicamycin treated yeast cells lost attachment capability to B. cinerea. To investigate contributions of attachment capability of R. glutinis to its biocontrol efficacy, yeast cells were mutagenized with 3% methane-sulfonic acid ethyl ester (EMS), and a mutant CE4 with stable non-attaching phenotype was obtained. No significant difference was found on colony, cell morphology, reproductive ability, and capsule formation between the mutant and wild-type. However, there was a distinct difference in India ink positive staining patterns between the two strains. Moreover, wild-type strain of R. glutinis showed better performance on inhibiting spore germination and mycelia' growth of B. cinerea than CE4 strain when yeast cells and B. cinerea were co-cultured in vitro. In biocontrol assay, both wild-type and CE4 strains showed significant biocontrol efficacy against gray mold caused by B. cinerea in apple fruit, whereas, control effect of CE4 strain was lower than that of wild-type. Our findings provided new evidences that attachment capability of R. glutinis to B. cinerea contributed to its biocontrol efficacy.
关键词	attachment biological control EMS mutagenization graymold yeast
学科领域	Microbiology
DOI	10.3389/fmicb.2016.00601
收录类别	SCI
语种	英语
WOS关键词	GREY MOLD DISEASE ; POSTHARVEST DISEASES ; BIOLOGICAL-CONTROL ; RHODOSPORIDIUM-TORULOIDES ; PENICILLIUM-EXPANSUM ; CRYPTOCOCCUS-LAURENTII ; PICHIA-GUILLIERMONDII ; POSSIBLE MODE ; APPLE FRUIT ; IN-VITRO
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000375394900001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3] ; Key Research Program of Chinese Academy of SciencesChinese Academy of Sciences [KITZD-SW-1.02] ; Youth Innovation Promotion Association CAS [2015063]
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25264
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Boqiang,Peng, Huaimin,Tian, Shiping. Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA	Li, Boqiang,Peng, Huaimin,&Tian, Shiping.(2016).Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy.FRONTIERS IN MICROBIOLOGY,7.
MLA	Li, Boqiang,et al."Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy".FRONTIERS IN MICROBIOLOGY 7(2016).

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