Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid)

doi:10.3389/fpls.2016.00491

	Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid)
	Zhu, Xiaopei 1,2; Chai, Min 1,2; Li, Yang 1; Sun, Meiyu 1; Zhang, Jinzheng 1; Sun, Guofeng 1; Jiang, Chuangdao 1; Shi, Lei1
	2016
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	7
摘要	As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Bb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.
关键词	lily dwarfism RNA-seq GA cell division cell expansion
学科领域	Plant Sciences
DOI	10.3389/fpls.2016.00491
收录类别	SCI
语种	英语
WOS关键词	GREEN-REVOLUTION ; CELL-CYCLE ; RNA-SEQ ; GIBBERELLIN BIOSYNTHESIS ; GENE ENCODES ; ARABIDOPSIS ; EXPRESSION ; RICE ; MUTANT ; PROTEINS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000374294700002
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Project of Ministry of Science and Technology of the People's Republic of China [863 program] [2011AA10020805] ; National Key Technology RD programNational Key Technology R&D Program [2012BAC01B05] ; Chinese Academy of SciencesChinese Academy of Sciences [CZBZX-1]
作者邮箱	shilei@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25265
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Xiaopei,Chai, Min,Li, Yang,et al. Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid)[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Zhu, Xiaopei.,Chai, Min.,Li, Yang.,Sun, Meiyu.,Zhang, Jinzheng.,...&Shi, Lei.(2016).Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid).FRONTIERS IN PLANT SCIENCE,7.
MLA	Zhu, Xiaopei,et al."Global Transcriptome Profiling Analysis of Inhibitory Effects of Paclobutrazol on Leaf Growth in Lily (Lilium Longiflorum-Asiatic Hybrid)".FRONTIERS IN PLANT SCIENCE 7(2016).

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