Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057

doi:10.1038/srep24066

	Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057
	Sun, Xiaoming 2,3; Zhao, Tingting 3; Gan, Shuheng ; Ren, Xiaodie ; Fang, Linchuan ; Karungo, Sospeter Karanja 3; Wang, Yi 2; Chen, Liang ; Li, Shaohua2 ; Xin, Haiping 1
	2016
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	Ethylene (ET) is a gaseous plant hormone that plays essential roles in biotic and abiotic stress responses in plants. However, the role of ET in cold tolerance varies in different species. This study revealed that low temperature promotes the release of ET in grapevine. The treatment of exogenous 1-aminocyclopropane-1-carboxylate increased the cold tolerance of grapevine. By contrast, the application of the ET biosynthesis inhibitor aminoethoxyvinylglycine reduced the cold tolerance of grapevine. This finding suggested that ET positively affected cold stress responses in grapevine. The expression of VaERF057, an ET signaling downstream gene, was strongly induced by low temperature. The overexpression of VaERF057 also enhanced the cold tolerance of Arabidopsis. Under cold treatment, malondialdehyde content was lower and superoxide dismutase, peroxidase, and catalase activities were higher in transgenic lines than in wild-type plants. RNA-Seq results showed that 32 stress-related genes, such as CBF1-3, were upregulated in VaERF057-overexpressing transgenic line. Yeast one-hybrid results further demonstrated that VaERF057 specifically binds to GCC-box and DRE motifs. Thus, VaERF057 may directly regulate the expression of its target stress-responsive genes by interacting with a GCC-box or a DRE element. Our work confirmed that ET positively regulates cold tolerance in grapevine by modulating the expression of VaERF057.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep24066
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTORS ; FREEZING TOLERANCE ; MOLECULAR CHARACTERIZATION ; LOW-TEMPERATURE ; GCC-BOX ; 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID ; PROTEASOMAL DEGRADATION ; CHILLING TOLERANCE ; STRESS TOLERANCE ; BINDING-PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373260600001
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31130047, 31471857] ; Grape Breeding Project of Ningxia [NXNYYZ201502] ; National Key Technology R&D Program of the Ministry of Science and TechnologyNational Key Technology R&D Program [2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS [2015281]
作者邮箱	shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com
作品OA属性	Green Published, gold
引用统计	被引频次：91[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25266
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sun, Xiaoming,Zhao, Tingting,Gan, Shuheng,et al. Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057[J]. SCIENTIFIC REPORTS,2016,6.
APA	Sun, Xiaoming.,Zhao, Tingting.,Gan, Shuheng.,Ren, Xiaodie.,Fang, Linchuan.,...&Xin, Haiping.(2016).Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057.SCIENTIFIC REPORTS,6.
MLA	Sun, Xiaoming,et al."Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057".SCIENTIFIC REPORTS 6(2016).

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