Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2

doi:10.1186/s12864-016-2576-7

	Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2
	Peng, Xianjun; Liu, Hui; Wang, Dan; Shen, Shihua
	2016
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	17
摘要	Background: The MYB family is one of the most abundant transcription factor families in plants. MYB proteins are involved in plant development, abiotic stress tolerance, hormone signal transduction and disease resistance. Here we perform genome-wide identification of MYB family transcription factors in an energy plant J. curcas, including determining family composition, phylogenetic evolution and functional prediction analysis. In addition, we further elucidate the function of the JcMYB2 gene. Methods: The phylogenetic trees were constructed by using the neighbor-joining method in MEGA 5.2. The biological functions of some JcMYBs were predicted according to orthology. The full length cDNA of JcMYB2 was cloned by using the RACE method. GUS histochemical staining was used to test the activity of the JcMYB2 promoter. Expression patterns of JcMYB2 were detected by using qPCR Transcriptional activity JcMYB2 were confirmed through yeast one hybrid. Subcellular Localization of JcMYB2 Protein were demonstrated by transient expression in the tobacco leaf. The function of JcMYB2 in salt and freezing tolerance were detected in transgenic plants. Results: A genome-wide analysis identified 128 MYB genes, including 123 R2R3-MYBs, 4 R1R2R3-MYBs and 1 4R-MYB. All of the R2R3-MYBs are further classified into 19 groups which indicated functional conservation among previously identified groups of R2R3-MYB proteins. Among of these newly identified MYBs, the JcMYB2 belongs to group G11 and its expression is induced obviously by cold, salt and MeJA (Methyl Jasmonate) and slightly by ABA (abscisic acid). JcMYB2 is localized to the nucleus and has transcriptional activity. JcMYB2 overexpressing plants are more tolerant to salt and cold stress than wild type plants. Tissue specific expression profiles showed that the JcMYB2 gene was expressed ubiquitously throughout the plant, with higher expression levels observed in the root. Conclusion: A comprehensive genome-wide analysis and phylogenetic relationship of R2R3-MYB subfamily in J. curcas present the global identification and functional prediction of JcR2R3-MYBs. Additionally, JcMYB2 regulates the stress response signaling networks by interacting with MeJA and ABA signaling pathway and functions in the root development of J. curcas.
关键词	Abiotic and biotic stress MYB transcription factor Promoter Tolerance
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s12864-016-2576-7
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR SUPERFAMILY ; EXPRESSION ANALYSIS ; SALT TOLERANCE ; COLD STRESS ; CELL-CYCLE ; ARABIDOPSIS ; PROTEINS ; DROUGHT ; RICE ; DOMAIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000372544000001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	Knowledge Innovation Program through Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-YW-359-2] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270653] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008]
作者邮箱	shshen@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25268
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xianjun,Liu, Hui,Wang, Dan,et al. Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2[J]. BMC GENOMICS,2016,17.
APA	Peng, Xianjun,Liu, Hui,Wang, Dan,&Shen, Shihua.(2016).Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2.BMC GENOMICS,17.
MLA	Peng, Xianjun,et al."Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2".BMC GENOMICS 17(2016).

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