Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants

doi:10.1111/pbi.12435

	Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants
	Gao, Qiong 1; Li, Xiaoxia ; Jia, Junting 1; Zhao, Pincang 1; Liu, Panpan 1; Liu, Zhujiang 1; Ge, Liangfa 2; Chen, Shuangyan ; Qi, Dongmei ; Deng, Bo 3; Lee, Byung-Hyun ; Liu, Gongshe; Cheng, Liqin
	2016
发表期刊	PLANT BIOTECHNOLOGY JOURNAL
ISSN	1467-7644
卷号	14 期号:3 页码:861-874
摘要	As a perennial forage crop broadly distributed in eastern Eurasia, sheepgrass (Leymus chinensis (Trin.) Tzvel) is highly tolerant to low-temperature stress. Previous report indicates that sheepgrass is able to endure as low as -47.5 degrees C,allowing it to survive through the cold winter season. However, due to the lack of sufficient studies, the underlying mechanism towards the extraordinary low-temperature tolerance is unclear. Although the transcription profiling has provided insight into the transcriptome response to cold stress, more detailed studies are required to dissect the molecular mechanism regarding the excellent abiotic stress tolerance. In this work, we report a novel transcript factor LcFIN1 (L.chinensis freezing-induced 1) from sheepgrass. LcFIN1 showed no homology with other known genes and was rapidly and highly induced by cold stress, suggesting that LcFIN1 participates in the early response to cold stress. Consistently, ectopic expression of LcFIN1 significantly increased cold stress tolerance in the transgenic plants, as indicated by the higher survival rate, fresh weight and other stress-related indexes after a freezing treatment. Transcriptome analysis showed that numerous stress-related genes were differentially expressed in LcFIN1-overexpressing plants, suggesting that LcFIN1 may enhance plant abiotic stress tolerance by transcriptional regulation. Electrophoretic mobility shift assays and CHIP-qPCR showed that LcCBF1 can bind to the CRT/DRE cis-element located in the promoter region of LcFIN1, suggesting that LcFIN1 is directly regulated by LcCBF1. Taken together, our results suggest that LcFIN1 positively regulates plant adaptation response to cold stress and is a promising candidate gene to improve crop cold tolerance.
关键词	cold tolerance LcFIN1 sheepgrass transcriptional activation
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1111/pbi.12435
收录类别	SCI
语种	英语
WOS关键词	SALT-INDUCED GENE ; BARLEY HVA1 GENE ; ARABIDOPSIS-THALIANA ; FREEZING TOLERANCE ; SIGNAL-TRANSDUCTION ; DROUGHT TOLERANCE ; ABIOTIC STRESS ; EXPRESSION ; PROTEIN ; SALINITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000371224700004
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1467-7652
资助机构	National Basic Research Program of China ('973')National Basic Research Program of China [2014CB138704] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170316] ; National High Technology Research and Development Program of China ('863')National High Technology Research and Development Program of China [2011AA100209] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2014ZX08009-003-002] ; Project of Ningxia 3 Agricultural Comprehensive Development Office [NTKJ-2014-04(1)]
作者邮箱	liugs@ibcas.ac.cn ; lqcheng@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25270
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK USA 4.China Agr Univ, Coll Anim Sci & Technol, Dept Grassland Sci, Beijing 100094, Peoples R China 5.Gyeongsang Natl Univ, PMBBRC, IALS, Div Appl Life Sci,Program BK21, Jinju, Gyeongsang, South Korea
推荐引用方式 GB/T 7714	Gao, Qiong,Li, Xiaoxia,Jia, Junting,et al. Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants[J]. PLANT BIOTECHNOLOGY JOURNAL,2016,14(3):861-874.
APA	Gao, Qiong.,Li, Xiaoxia.,Jia, Junting.,Zhao, Pincang.,Liu, Panpan.,...&Cheng, Liqin.(2016).Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants.PLANT BIOTECHNOLOGY JOURNAL,14(3),861-874.
MLA	Gao, Qiong,et al."Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants".PLANT BIOTECHNOLOGY JOURNAL 14.3(2016):861-874.

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