Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

doi:10.1007/s00299-015-1910-x

	Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis
	Yuan, Yangyang 1; Fang, Linchuan 1; Karungo, Sospeter Karanja 1; Zhang, Langlang 1; Gao, Yingying 1; Li, Shaohua2,3 ; Xin, Haiping 2,3
	2016
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	35 期号:3 页码:655-666
摘要	The plant-specific GRAS transcription factor family regulates diverse processes involved in plant growth, development and stress responses. In this study, VaPAT1, a GRAS gene from Vitis amurensis was isolated and functionally characterized. Sequence alignment and phylogenetic analysis showed that VaPAT1 has a high sequence identity to CmsGRAS and OsCIGR1, which belong to PAT1 branch of GRAS family and function in stress resistance. The transcription of VaPAT1 was markedly induced by stress-related phytohormone abscisic acid (ABA) and various abiotic stress treatments such as cold, drought and high salinity, however, it was repressed by exogenous gibberellic acid (GA) application. Overexpression of VaPAT1 increased the cold, drought and high salinity tolerance in transgenic Arabidopsis. When compared with wild type (WT) seedlings, the VaPAT1-overexpression lines accumulated higher levels of proline and soluble sugar under these stress treatments. Moreover, stress-related genes such as AtSIZ1, AtCBF1, AtATR1/MYB34, AtMYC2, AtCOR15A, AtRD29A and AtRD29B showed higher expression levels in VaPAT1 transgenic lines than in WT Arabidopsis under normal growth conditions. Together, our results indicated that VaPAT1 functions as a positive transcriptional regulator involved in grapevine abiotic stress responses.
关键词	GRAS transcription factor VaPAT1 Vitis amurensis Abiotic stress Gibberellic acid (GA) Stress-related genes
学科领域	Plant Sciences
DOI	10.1007/s00299-015-1910-x
收录类别	SCI
语种	英语
WOS关键词	SIGNAL-TRANSDUCTION ; ABSCISIC-ACID ; DROUGHT TOLERANCE ; COLD TOLERANCE ; GENE FAMILY ; GIBBERELLIN RESPONSES ; EXPRESSION ANALYSIS ; TRANSGENIC RICE ; SALT STRESSES ; THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000371241100013
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-203X
资助机构	National Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31130047, 31471857] ; National Key Technology R&D Program of the Ministry of Science and Technology during the Twelfth Five-year Plan Period [2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS [2015281]
作者邮箱	xinhaiping215@hotmail.com
引用统计	被引频次：82[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25272
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Yangyang,Fang, Linchuan,Karungo, Sospeter Karanja,et al. Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis[J]. PLANT CELL REPORTS,2016,35(3):655-666.
APA	Yuan, Yangyang.,Fang, Linchuan.,Karungo, Sospeter Karanja.,Zhang, Langlang.,Gao, Yingying.,...&Xin, Haiping.(2016).Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis.PLANT CELL REPORTS,35(3),655-666.
MLA	Yuan, Yangyang,et al."Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis".PLANT CELL REPORTS 35.3(2016):655-666.

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