Engineering metal-binding sites of bacterial CusF to enhance Zn/Cd accumulation and resistance by subcellular targeting

doi:10.1016/j.jhazmat.2015.09.054

	Engineering metal-binding sites of bacterial CusF to enhance Zn/Cd accumulation and resistance by subcellular targeting
	Yu, Pengli ; Yuan, Jinhong ; Zhang, Hui 1; Deng, Xin2,3 ; Ma, Mi ; Zhang, Haiyan
	2016
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	302 页码:275-285
摘要	The periplasmic protein CusF acts as a metallochaperone to mediate Cu resistance in Escherichia coli. CusF does not contain cysteine residues and barely binds to divalent cations. Here, we addressed effects of cysteine-substitution mutant (named as mCusF) of CusF on zinc/cadmium (Zn/Cd) accumulation and resistance. We targeted mCusF to different subcellular compartments in Arabidopsis. We found that plants expressing vacuole-targeted mCusF were more resistant to excess Zn than WT and plants with cell wall-targeted or cytoplasmic mCusF. Under long-term exposure to excess Zn, all transgenic lines accumulated more Zn (up to 2.3-fold) in shoots than the untransformed plants. Importantly, plants with cytoplasmic mCusF showed higher efficiency of Zn translocation from root to shoot than plants with secretory pathway-targeted-mCusF. Furthermore, the transgenic lines exhibited enhanced resistance to Cd and significant increase in root-to-shoot Cd translocation. We also found all transgenic plants greatly improved manganese (Mn) and iron (Fe) homeostasis under Cd exposure. Our results demonstrate heterologous expression of mCusF could be used to engineer a new phytoremediation strategy for Zn/Cd and our finding also deepen our insights into mechanistic basis for relieving Cd toxicity in plants through proper root/shoot partitioning mechanism and homeostatic accumulation of Mn and Fe. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Cadmium (Cd) CusF Cysteine Subcellular targeting Zinc (Zn)
学科领域	Engineering, Environmental ; Environmental Sciences
DOI	10.1016/j.jhazmat.2015.09.054
收录类别	SCI
语种	英语
WOS关键词	THLASPI-CAERULESCENS ; HIGH-AFFINITY ; CADMIUM ; ZINC ; ARABIDOPSIS ; TRANSPORT ; ROOT ; TOLERANCE ; SHOOT ; ZN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000366225300033
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-3336
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270313, 31470292]
作者邮箱	hyz@ibcas.ac.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25276
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 4.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Yu, Pengli,Yuan, Jinhong,Zhang, Hui,et al. Engineering metal-binding sites of bacterial CusF to enhance Zn/Cd accumulation and resistance by subcellular targeting[J]. JOURNAL OF HAZARDOUS MATERIALS,2016,302:275-285.
APA	Yu, Pengli,Yuan, Jinhong,Zhang, Hui,Deng, Xin,Ma, Mi,&Zhang, Haiyan.(2016).Engineering metal-binding sites of bacterial CusF to enhance Zn/Cd accumulation and resistance by subcellular targeting.JOURNAL OF HAZARDOUS MATERIALS,302,275-285.
MLA	Yu, Pengli,et al."Engineering metal-binding sites of bacterial CusF to enhance Zn/Cd accumulation and resistance by subcellular targeting".JOURNAL OF HAZARDOUS MATERIALS 302(2016):275-285.

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