Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries

doi:10.1007/s00425-015-2391-4

	Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries
	Guan, Le ; Dai, Zhanwu; Wu, Ben-Hong ; Wu, Jing ; Merlin, Isabelle ; Hilbert, Ghislaine ; Renaud, Christel ; Gomes, Eric ; Edwards, Everard 3; Li, Shao-Hua; Delrot, Serge
	2016
发表期刊	PLANTA
ISSN	0032-0935
卷号	243 期号:1 页码:23-41
摘要	Light exclusion reduces the concentration and modifies the composition of grape anthocyanins, by altering the expression of genes involved in anthocyanin biosynthesis and transport, in a cultivar- and tissue-specific manner. Unlike most grapes, teinturier grapes accumulate anthocyanins both in skin and flesh. However, the concentration and composition of anthocyanins in both tissues differ, providing a valuable system to study tissue-specific regulation of anthocyanin synthesis. Furthermore, little is known about the mechanisms controlling the sensitivity of anthocyanin accumulation to light. Here, light was excluded from Gamay (white-fleshed) and Gamay Fr,aux (teinturier mutant) berries throughout berry development. Under light-exposed conditions, the skin of Gamay Fr,aux accumulated the highest level of anthocyanins, followed by the skin of Gamay, while the pulp of Gamay Fr,aux had much lower anthocyanins than the skins. Network analysis revealed the same order on the number of significant correlations among metabolites and transcripts in the three colored tissues, indicating a higher connectivity that reflects a higher efficiency of the anthocyanin pathway. Compared to light conditions, light exclusion reduced the total amount of anthocyanins, most severely in the skin of Gamay and to a lesser extent in the flesh and skin of Gamay Fr,aux. Coordinated decrease in the transcript abundance of structural, regulatory and transporter genes by light exclusion correlated with the reduced anthocyanin concentration in a cultivar- and tissue-specific manner. Moreover, light exclusion increased the ratio of dihydroxylated to trihydroxylated anthocyanins, in parallel with F3'H and F3'5'H transcript amounts. Sugars and ABA only play a limited role in the control of anthocyanin synthesis in the berries, in contrast with what has been described in cell suspensions. This study provides novel insights into the regulation of anthocyanin in wild type and teinturier cultivars.
关键词	Fruit color Gamay Freaux Ripening Secondary metabolism Solar radiation Vitis
学科领域	Plant Sciences
DOI	10.1007/s00425-015-2391-4
收录类别	SCI
语种	英语
WOS关键词	VITIS-VINIFERA L. ; SUCROSE-SPECIFIC INDUCTION ; CELL-SUSPENSION CULTURES ; FLAVONOID BIOSYNTHESIS ; GENE-EXPRESSION ; TRANSCRIPTION FACTOR ; SOLAR-RADIATION ; RED-COLOR ; ACCUMULATION ; SUNLIGHT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000367614800003
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2048
作者邮箱	zhanwu.dai@bordeaux.inra.fr
引用统计	被引频次：85[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25277
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Bordeaux, ISVV, INRA, UMR EGFV 1287, F-33882 Villenave Dornon, France 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 4.CSIRO, Agr Flagship, Glen Osmond, SA 5064, Australia
推荐引用方式 GB/T 7714	Guan, Le,Dai, Zhanwu,Wu, Ben-Hong,et al. Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries[J]. PLANTA,2016,243(1):23-41.
APA	Guan, Le.,Dai, Zhanwu.,Wu, Ben-Hong.,Wu, Jing.,Merlin, Isabelle.,...&Delrot, Serge.(2016).Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries.PLANTA,243(1),23-41.
MLA	Guan, Le,et al."Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries".PLANTA 243.1(2016):23-41.

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