An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake

doi:10.1111/nph.13637

	An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake
	He, Zhenyan; Yan, Huili 1; Chen, Yanshan 1; Shen, Hongling 1; Xu, Wenxiu ; Zhang, Haiyan ; Shi, Lei; Zhu, Yong-Guan ; Ma, Mi
	2016
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	209 期号:2 页码:746-761
摘要	The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As (III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake. PvTIP4;1 was identified from yeast functional complement cDNA library of P. vittata. Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis. Subcellular localization of PvTIP4;1-GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4; 1 was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied. Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittata cells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site. PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin from P. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.
关键词	aromatic/arginine (Ar/R) arsenic (As) arsenite (As(III)) aquaporin Pteris vittata PvTIP4 1 uptake
学科领域	Plant Sciences
DOI	10.1111/nph.13637
收录类别	SCI
语种	英语
WOS关键词	MAJOR INTRINSIC PROTEINS ; ARSENATE REDUCTASE ; VACUOLAR MEMBRANE ; ARABIDOPSIS ; GLYCEROL ; FERN ; SELECTIVITY ; TRANSPORT ; FAMILY ; CHANNEL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373376500029
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1469-8137
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370299, 31470549]
作者邮箱	ygzhu@iue.ac.cn ; mami@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：76[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25279
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China
推荐引用方式 GB/T 7714	He, Zhenyan,Yan, Huili,Chen, Yanshan,et al. An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake[J]. NEW PHYTOLOGIST,2016,209(2):746-761.
APA	He, Zhenyan.,Yan, Huili.,Chen, Yanshan.,Shen, Hongling.,Xu, Wenxiu.,...&Ma, Mi.(2016).An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake.NEW PHYTOLOGIST,209(2),746-761.
MLA	He, Zhenyan,et al."An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake".NEW PHYTOLOGIST 209.2(2016):746-761.

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