Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily

doi:10.1186/s12864-016-3226-9

	Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily
	Wu, Qian ; Wu, Jie ; Li, Shan-Shan ; Zhang, Hui-Jin ; Feng, Cheng-Yong ; Yin, Dan-Dan ; Wu, Ru-Yan ; Wang, Liang-Sheng
	2016
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	17
摘要	Background: Waterlily (Nymphaea spp.), a perennial herbaceous aquatic plant, is divided into two ecological groups: hardy waterlily and tropical waterlily. Although the hardy waterlily has no attractive blue flower cultivar, its adaptability is stronger than tropical waterlily because it can survive a cold winter. Thus, breeding hardy waterlily with real blue flowers has become an important target for breeders. Molecular breeding may be a useful way. However, molecular studies on waterlily are limited due to the lack of sequence data. Results: In this study, six cDNA libraries generated from the petals of two different coloring stages of blue tropical waterlily cultivar Nymphaea 'King of Siam' were sequenced using the Illumina HiSeq (TM) 2500 platform. Each library produced no less than 5.65 Gb clean reads. Subsequently, de novo assembly generated 112,485 unigenes, including 26,206 unigenes annotated to seven public protein databases. Then, 127 unigenes could be identified as putative homologues of color-related genes in other species, including 28 up-regulated and 5 down-regulated unigenes. In petals, 16 flavonoids (4 anthocyanins and 12 flavonols) were detected in different contents during the color development due to the different expression levels of color-related genes, and four flavonols were detected in waterlily for the first time. Furthermore, UA3GTs were selected as the most important candidates involved in the flavonoid metabolic pathway, UA3GTs induced blue petal color formation in Nymphaea 'King of Siam'. Conclusions: This study will improve our understanding of the molecular mechanism of blue flowers in waterlily and provide the basis for molecular breeding of blue hardy waterlily cultivars.
关键词	Waterlily Transcriptome Anthocyanin Flavonoid Blue color UAGT
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s12864-016-3226-9
收录类别	SCI
语种	英语
WOS关键词	INDUCED DIABETIC-RATS ; RNA-SEQ DATA ; ANTHOCYANIN BIOSYNTHESIS ; GENETIC-MODIFICATION ; PHENOLIC-COMPOUNDS ; NYMPHAEA-CAERULEA ; REFERENCE GENOME ; UDP-GLUCOSE ; DELPHINIDIN ; COLOR
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000387428000001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471910]
作者邮箱	shshli@ibcas.ac.cn ; wanglsh@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25282
专题	中科院北方资源植物重点实验室
作者单位	1.[Wu, Qian 2.Wu, Jie 3.Li, Shan-Shan 4.Zhang, Hui-Jin 5.Feng, Cheng-Yong 6.Yin, Dan-Dan 7.Wu, Ru-Yan 8.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 9.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 11.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wu, Qian,Wu, Jie,Li, Shan-Shan,et al. Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily[J]. BMC GENOMICS,2016,17.
APA	Wu, Qian.,Wu, Jie.,Li, Shan-Shan.,Zhang, Hui-Jin.,Feng, Cheng-Yong.,...&Wang, Liang-Sheng.(2016).Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily.BMC GENOMICS,17.
MLA	Wu, Qian,et al."Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily".BMC GENOMICS 17(2016).

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