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CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.) | |
Ren, Chong1,2; Liu, Xianju1,2; Zhang, Zhan1,2; Wang, Yi1,2; Duan, Wei1; Li, Shaohua1; Liang, Zhenchang1,3 | |
2016 | |
发表期刊 | SCIENTIFIC REPORTS |
ISSN | 2045-2322 |
卷号 | 6 |
摘要 | The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in 'Chardonnay' suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1-to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape. |
学科领域 | Multidisciplinary Sciences |
DOI | 10.1038/srep32289 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | CRISPR-CAS SYSTEM ; RNA-GUIDED ENDONUCLEASE ; GENETIC-TRANSFORMATION ; GENOME MODIFICATIONS ; TRANSGENIC PLANTS ; LATER GENERATIONS ; ANALYSIS REVEALS ; 1ST GENERATION ; NUCLEASES ; RICE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000382239600001 |
出版者 | NATURE PUBLISHING GROUP |
文献子类 | Article |
出版地 | LONDON |
资助机构 | National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572090] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] ; Hundred Talents of Chinese Academy of Sciences |
作者邮箱 | ZL249@ibcas.ac.cn |
作品OA属性 | Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25284 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China |
推荐引用方式 GB/T 7714 | Ren, Chong,Liu, Xianju,Zhang, Zhan,et al. CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Ren, Chong.,Liu, Xianju.,Zhang, Zhan.,Wang, Yi.,Duan, Wei.,...&Liang, Zhenchang.(2016).CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.).SCIENTIFIC REPORTS,6. |
MLA | Ren, Chong,et al."CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)".SCIENTIFIC REPORTS 6(2016). |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Ren-2016-CRISPR_Cas9(3116KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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