Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.)

doi:10.1186/s12864-016-2989-3

	Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.)
	Ren, Chong 1,2; Zhang, Zhan 1,2; Wang, Yi 1,2; Li, Shaohua1 ; Liang, Zhenchang 1
	2016
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	17
摘要	Background: Nuclear factor Y (NF-Y) transcription factor is composed of three distinct subunits: NF-YA, NF-YB and NF-YC. Many members of NF-Y family have been reported to be key regulators in plant development, phytohormone signaling and drought tolerance. However, the function of the NF-Y family is less known in grape (Vitis vinifera L.). Results: A total of 34 grape NF-Y genes that distributed unevenly on grape (V. vinifera) chromosomes were identified in this study. Phylogenetic analysis was performed to predict functional similarities between Arabidopsis thaliana and grape NF-Y genes. Comparison of the structures of grape NF-Y genes (VvNF-Ys) revealed their functional conservation and alteration. Furthermore, we investigated the expression profiles of VvNF-Ys in response to various stresses, phytohormone treatments, and in leaves and grape berries with various sugar contents at different developmental stages. The relationship between VvNF-Y transcript levels and sugar content was examined to select candidates for exogenous sugar treatments. Quantitative real-time PCR (qPCR) indicated that many VvNF-Ys responded to different sugar stimuli with variations in transcript abundance. qPCR and publicly available microarray data suggest that VvNF-Ys exhibit distinct expression patterns in different grape organs and developmental stages, and a number of VvNF-Ys may participate in responses to multiple abiotic and biotic stresses, phytohormone treatments and sugar accumulation or metabolism. Conclusions: In this study, we characterized 34 VvNF-Ys based on their distributions on chromosomes, gene structures, phylogenetic relationship with Arabidopsis NF-Y genes, and their expression patterns. The potential roles of VvNF-Ys in sugar accumulation or metabolism were also investigated. Altogether, the data provide significant insights on VvNF-Ys, and lay foundations for further functional studies of NF-Y genes in grape.
关键词	Grape (Vitis vinifera L.) NF-Y transcription factor Phylogenetic analysis Expression profiles Quantitative real-time PCR
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s12864-016-2989-3
收录类别	SCI
语种	英语
WOS关键词	CCAAT-BINDING FACTOR ; SEED OIL PRODUCTION ; TRANSCRIPTION FACTOR ; FACTOR CBF ; SOMATIC EMBRYOGENESIS ; FUNCTIONAL DOMAINS ; LEAFY COTYLEDON1 ; STRESS-RESPONSE ; DNA COMPLEX ; B-SUBUNIT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000384046400001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572090]
作者邮箱	shhli@ibcas.ac.cn ; ZL249@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25285
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ren, Chong,Zhang, Zhan,Wang, Yi,et al. Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.)[J]. BMC GENOMICS,2016,17.
APA	Ren, Chong,Zhang, Zhan,Wang, Yi,Li, Shaohua,&Liang, Zhenchang.(2016).Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.).BMC GENOMICS,17.
MLA	Ren, Chong,et al."Genome-wide identification and characterization of the NF-Y gene family in grape (vitis vinifera L.)".BMC GENOMICS 17(2016).

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