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| Expression of Vitis amurensis NAC26 in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis | |
Fang, Linchuan1; Su, Lingye; Sun, Xiaoming1; Li, Xinbo2; Sun, Mengxiang2; Karungo, Sospeter Karanja1; Fang, Shuang3; Chu, Jinfang3; Li, Shaohua ; Xin, Haiping1
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| 2016 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
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| ISSN | 0022-0957 |
| Volume | 67Issue:9Pages:2829-2845 |
| Abstract | The growth and fruit quality of grapevines are widely affected by abnormal climatic conditions such as water deficits, but many of the precise mechanisms by which grapevines respond to drought stress are still largely unknown. Here, we report that VaNAC26, a member of the NAC transcription factor family, was upregulated dramatically during cold, drought and salinity treatments in Vitis amurensis, a cold and drought-hardy wild Vitis species. Heterologous overexpression of VaNAC26 enhanced drought and salt tolerance in transgenic Arabidopsis. Higher activities of antioxidant enzymes and lower concentrations of H2O2 and O-2(-) were found in VaNAC26-OE lines than in wild type plants under drought stress. These results indicated that scavenging by reactive oxygen species (ROS) was enhanced by VaNAC26 in transgenic lines. Microarray-based transcriptome analysis revealed that genes related to jasmonic acid (JA) synthesis and signaling were upregulated in VaNAC26-OE lines under both normal and drought conditions. VaNAC26 showed a specific binding ability on the NAC recognition sequence (NACRS) motif, which broadly exists in the promoter regions of upregulated genes in transgenic lines. Endogenous JA content significantly increased in the VaNAC26-OE lines 2 and 3. Our data suggest that VaNAC26 responds to abiotic stresses and may enhance drought tolerance by transcriptional regulation of JA synthesis in Arabidopsis. |
| Keyword | Drought stress JA biosynthesis ROS transcriptional regulation VaNAC26 Vitis amurensis |
| Subject Area | Plant Sciences |
| DOI | 10.1093/jxb/erw122 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | NAC TRANSCRIPTION FACTOR ; STRESS TOLERANCE ; ABSCISIC-ACID ; LEAF SENESCENCE ; WATER-STRESS ; GENE FAMILY ; COLD ; DEHYDRATION ; RESPONSES ; DEFENSE |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000376385800026 |
| Publisher | OXFORD UNIV PRESS |
| Subtype | Article |
| Publication Place | OXFORD |
| EISSN | 1460-2431 |
| Funding Organization | National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31130047, 91117016] ; National Key Technology R&D Program of the Ministry of Science and TechnologyNational Key Technology R&D Program [2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS [2015281] |
| Corresponding Author Email | shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com |
| OA | hybrid, Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25287 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Wuhan Univ, State Key Lab Plant Hybrid Rice, Coll Life Sci, Dept Cell & Dev Biol, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Fang, Linchuan,Su, Lingye,Sun, Xiaoming,et al. Expression of Vitis amurensis NAC26 in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(9):2829-2845. |
| APA | Fang, Linchuan.,Su, Lingye.,Sun, Xiaoming.,Li, Xinbo.,Sun, Mengxiang.,...&Xin, Haiping.(2016).Expression of Vitis amurensis NAC26 in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis.JOURNAL OF EXPERIMENTAL BOTANY,67(9),2829-2845. |
| MLA | Fang, Linchuan,et al."Expression of Vitis amurensis NAC26 in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis".JOURNAL OF EXPERIMENTAL BOTANY 67.9(2016):2829-2845. |
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| Fang-2016-Expression（10599KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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