Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803

doi:10.1002/1873-3468.12416

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	Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803
	Dong, Wei 1; Wang, Jia 1; Niu, Guoqi 2; Zhao, Shun ; Liu, Lin
	2016
发表期刊	FEBS LETTERS
ISSN	0014-5793
卷号	590 期号:19 页码:3435-3442
摘要	The high temperature requirement A (HtrA) proteases are oligomeric serine proteases essential for protein quality control. HtrA homolog A (HhoA) from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 assembles into a proteolytically active hexamer. Herein, we present the crystal structure of the hexameric HhoA in complex with the copurified peptide. Our data indicate the presence of three methionines in close proximity to the peptide-binding site of the PDZ domain. Unexpectedly, we observed that a zinc ion is accommodated within the central channel formed by a HhoA trimer. However, neither calcium nor magnesium showed affinity for HhoA. The role of the zinc ion in HhoA was tested in an in vitro proteolytic assay against the nonspecific substrate -casein and was found to be inhibitory. Our findings provide insights into the regulation of HhoA by a redox-related mechanism involving methionine residues and by zinc ion-binding within the central channel.
关键词	HtrA protease oligomerization PDZ domain redox regulation zinc binding
学科领域	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
DOI	10.1002/1873-3468.12416
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYSTEM-II ; DEG/HTRA PROTEASES ; QUALITY CONTROL ; HTRA PROTEASES ; DEG PROTEASES ; CELL ; FAMILY ; DISTINCT ; REPAIR ; ADAPTATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000386153900013
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1873-3468
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170688]
作者邮箱	liulin@ibcas.ac.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25293
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Dong, Wei,Wang, Jia,Niu, Guoqi,et al. Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803[J]. FEBS LETTERS,2016,590(19):3435-3442.
APA	Dong, Wei,Wang, Jia,Niu, Guoqi,Zhao, Shun,&Liu, Lin.(2016).Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803.FEBS LETTERS,590(19),3435-3442.
MLA	Dong, Wei,et al."Crystal structure of the zinc-bound HhoA protease from Synechocystis sp PCC 6803".FEBS LETTERS 590.19(2016):3435-3442.

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