CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex

doi:10.1093/pcp/pcw130

IB-CAS > 中科院光生物学重点实验室

	CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex
	Yamamoto, Hiroshi 1; Fan, Xiangyuan 2; Sugimoto, Kazuhiko ; Fukao, Yoichiro 3,4; Peng, Lianwei 2; Shikanai, Toshiharu 1
	2016
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	57 期号:10 页码:2122-2132
摘要	In vascular plants, the chloroplast NADH dehydrogenase-like (NDH) complex, a homolog of respiratory NADH: quinone oxidoreductase (Complex I), mediates plastoquinone reduction using ferredoxin as an electron donor in cyclic electron transport around PSI in the thylakoid membrane. In angiosperms, chloroplast NDH is composed of five subcomplexes and forms a supercomplex with PSI. The modular assembly of stroma-protruded subcomplex A, which corresponds to the Q module of Complex I, was recently reported. However, the factors involved in the specific assembly steps have not been completely identified. Here, we isolated an Arabidopsis mutant, chlororespiratory reduction 9 (crr9), defective in NDH activity. The CRR9 gene encodes a novel stromal protein without any known functional domains or motifs. CRR9 is highly conserved in cyanobacteria and land plants but not in green algae, which do not have chloroplast NDH. Blue native-PAGE and immunoblot analyses of thylakoid proteins indicated that formation of subcomplex A was impaired in crr9. CRR9 was specifically required for the accumulation of NdhK, a subcomplex A subunit, in NDH assembly intermediates in the stroma. Furthermore, two-dimensional clear native/SDS-PAGE analysis of the stroma fraction indicated that incorporation of NdhM into NDH assembly intermediate complex 400 was impaired in crr9. These results suggest that CRR9 is a novel factor required for the formation of NDH subcomplex A.
关键词	Arabidopsis Assembly factor Chloroplast Chloroplast NDH Cyclic electron transport around PSI
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcw130
收录类别	SCI
语种	英语
WOS关键词	CYCLIC ELECTRON-TRANSPORT ; DOMAIN-LIKE FOLD ; PHOTOSYSTEM-I ; NAD(P)H DEHYDROGENASE ; RESPIRATORY COMPLEX ; PHYSIOLOGICAL-ROLE ; ARABIDOPSIS-THALIANA ; FLUCTUATING LIGHT ; QUANTUM YIELD ; TOBACCO
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000388374100009
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	Japan Science and Technology AgencyJapan Science & Technology Agency (JST) ; Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [25251032] ; Network of Centers of Carbon Dioxide Resource Studies in Plants ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270289]
作者邮箱	shikanai@pmg.bot.kyoto-u.ac.jp
作品OA属性	Bronze
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25295
专题	中科院光生物学重点实验室
作者单位	1.Kyoto Univ, Grad Sch Sci, Dept Bot, Kyoto 6068502, Japan 2.Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020076, Japan 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 4.Ritsumeikan Univ, Dept Bioinformat, Kusatsu, Shiga 5258577, Japan 5.Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan
推荐引用方式 GB/T 7714	Yamamoto, Hiroshi,Fan, Xiangyuan,Sugimoto, Kazuhiko,et al. CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex[J]. PLANT AND CELL PHYSIOLOGY,2016,57(10):2122-2132.
APA	Yamamoto, Hiroshi,Fan, Xiangyuan,Sugimoto, Kazuhiko,Fukao, Yoichiro,Peng, Lianwei,&Shikanai, Toshiharu.(2016).CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex.PLANT AND CELL PHYSIOLOGY,57(10),2122-2132.
MLA	Yamamoto, Hiroshi,et al."CHLORORESPIRATORY REDUCTION 9 is a Novel Factor Required for Formation of Subcomplex A of the Chloroplast NADH Dehydrogenase-Like Complex".PLANT AND CELL PHYSIOLOGY 57.10(2016):2122-2132.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Yamamoto-2016-CHLORO（2483KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文