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| Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 | |
| Zhao, Shun1; Liu, Lin | |
| 2016 | |
| Source Publication | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| Volume | 72Pages:795-798 |
| Abstract | GDP-D-mannose pyrophosphorylase catalyzes the production of GDP-D-mannose, an intermediate product in the plant ascorbic acid (AsA) biosynthetic pathway. This enzyme is a key regulatory target in AsA biosynthesis and is encoded by VITAMIN C DEFECTIVE 1 (VTC1) in the Arabidopsis thaliana genome. Here, recombinant VTC1 was expressed, purified and crystallized. Diffraction data were obtained from VTC1 crystals grown in the absence and presence of substrate using X-rays. The ligand-free VTC1 crystal diffracted X-rays to 3.3 angstrom resolution and belonged to space group R32, with unit-cell parameters a = b = 183.6, c = 368.5 angstrom, alpha = beta = 90, gamma = 120 degrees; the crystal of VTC1 in the presence of substrate diffracted X-rays to 1.75 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 70.8, b = 83.9, c = 74.5 angstrom, alpha = gamma = 90.0, beta = 114.9 degrees. |
| Keyword | ascorbic acid biosynthesis GDP-D-mannose pyrophosphorylase Arabidopsis thaliana |
| Subject Area | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| DOI | 10.1107/S2053230X16013406 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ASCORBIC-ACID SYNTHESIS ; VITAMIN-C ; BIOSYNTHESIS ; METABOLISM ; DEFICIENT ; MUTANT ; PLANTS |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000387585000010 |
| Publisher | INT UNION CRYSTALLOGRAPHY |
| Subtype | Article |
| Publication Place | CHESTER |
| EISSN | 2053-230X |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170688] |
| Corresponding Author Email | zhaoshun2010@ibcas.ac.cn |
| OA | Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25296 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Shun,Liu, Lin. Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2016,72:795-798. |
| APA | Zhao, Shun,&Liu, Lin.(2016).Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,72,795-798. |
| MLA | Zhao, Shun,et al."Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 72(2016):795-798. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Zhao-2016-Expression（606KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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