Structural basis for copper/silver binding by the Synechocystis metallochaperone CopM

doi:10.1107/S2059798316011943

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	Structural basis for copper/silver binding by the Synechocystis metallochaperone CopM
	Zhao, Shun 1; Wang, Xiao 1; Niu, Guoqi 2; Dong, Wei 1; Wang, Jia 1; Fang, Ying 1; Lin, Yajing 3; Liu, Lin
	2016
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
ISSN	2059-7983
卷号	72 页码:997-1005
摘要	Copper homeostasis integrates multiple processes from sensing to storage and efflux out of the cell. CopM is a cyanobacterial metallochaperone, the gene for which is located upstream of a two-component system for copper resistance, but the molecular basis for copper recognition by this four-helical bundle protein is unknown. Here, crystal structures of CopM in apo, copper-bound and silver-bound forms are reported. Monovalent copper/silver ions are buried within the bundle core; divalent copper ions are found on the surface of the bundle. The monovalent copper/silver-binding site is constituted by two consecutive histidines and is conserved in a previously functionally unknown protein family. The structural analyses show two conformational states and suggest that flexibility in the first alpha-helix is related to the metallochaperone function. These results also reveal functional diversity from a protein family with a simple fourhelical fold.
关键词	copper homeostasis copper-binding protein metal coordination helical bundle
学科领域	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
DOI	10.1107/S2059798316011943
收录类别	SCI
语种	英语
WOS关键词	SP PCC 6803 ; COPPER RESISTANCE ; CYANOBACTERIAL METALLOCHAPERONE ; MACROMOLECULAR CRYSTALLOGRAPHY ; 2-COMPONENT PROTEINS ; STRUCTURE REFINEMENT ; CENTRAL METABOLISM ; GENE-CLUSTER ; METAL ; HOMEOSTASIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000383998200002
出版者	INT UNION CRYSTALLOGRAPHY
文献子类	Article
出版地	CHESTER
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2011CBA00901] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170688] ; Chinese Academy of SciencesChinese Academy of Sciences [KGZD-EW-T05]
作者邮箱	liulin@ibcas.ac.cn
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25300
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Lab Noncoding RNA, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Shun,Wang, Xiao,Niu, Guoqi,et al. Structural basis for copper/silver binding by the Synechocystis metallochaperone CopM[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2016,72:997-1005.
APA	Zhao, Shun.,Wang, Xiao.,Niu, Guoqi.,Dong, Wei.,Wang, Jia.,...&Liu, Lin.(2016).Structural basis for copper/silver binding by the Synechocystis metallochaperone CopM.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,72,997-1005.
MLA	Zhao, Shun,et al."Structural basis for copper/silver binding by the Synechocystis metallochaperone CopM".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72(2016):997-1005.

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