Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions

doi:10.1007/s12374-016-0117-1

IB-CAS > 中科院光生物学重点实验室

	Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions
	Li, Lingyu 1; Yang, Haomeng ; Ren, Weibo 2; Liu, Bei 1; Cheng, Dongmei 1; Wu, Xinhong 2; Gong, Jirui 3; Peng, Lianwei ; Huang, Fang
	2016
发表期刊	JOURNAL OF PLANT BIOLOGY
ISSN	1226-9239
卷号	59 期号:4 页码:336-346
摘要	Sheepgrass [Leymus chinensis (Trin.) Tzvel] is a valuble forage plant highly significant to regional grassland productivity of Euro-Asia steppes. Although effects of environmental stress including drought have been studied, impact of nutrient deficiency in particular phosphate (Pi), one of the essential macronutrient, is not reported. Here, we investigated low-Pi effect on its photosynthetic apparatus via physiological and biochemical analysis. We show that PSII activity was significantly reduced based on chlorophyll fluorescence measurements. We observed decreased amount of core proteins of PSII by immunoblot analysis. Further analysis of thylakoid membranes using 2D-BN/SDS-PAGE and immunoblot detection demonstrated that the amount of PSII complexes was closely correlated with the Pi levels within the range. Together with reduced number of thylakoid grana stackings observed, we suggest that the maintenance of PSII is impaired under Pi-limited condition. Moreover, enzyme activity assays revealed that the activity of several ROS scavenger enzymes was stimulated by low-Pi treatment. Based on these experimental results we conclude that PSII is the component of photosynthetic apparatus most sensitive to Pi supply and the enhanced anti-ROS activity is mainly subjective to protection of PSII against low Pi-induced photo-oxidative stress in the organism.
关键词	Leymus chinensis Phosphate starvation Photosystem II ROS-scavenging enzymes Thylakoid membranes 2D-BN/PAGE
学科领域	Plant Sciences
DOI	10.1007/s12374-016-0117-1
收录类别	SCI
语种	英语
WOS关键词	PHOTOOXIDATIVE STRESS ; MOLECULAR-MECHANISMS ; PHOSPHORUS-NUTRITION ; LIPID-PEROXIDATION ; PHOTOSYSTEM-II ; GLYCINE-MAX ; ARABIDOPSIS ; PLANTS ; TOLERANCE ; ASSAY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000381137700004
出版者	SPRINGER HEIDELBERG
文献子类	Article
出版地	HEIDELBERG
EISSN	1867-0725
资助机构	National Key Basic Research Development Program of ChinaNational Basic Research Program of China [2014CB138800] ; Key Research Program of the Chinese Academy of Science [KGZD-EW-T05]
作者邮箱	fhuang@ibcas.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25302
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China 4.Beijing Normal Univ, Coll Resources Sci & Technol, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Li, Lingyu,Yang, Haomeng,Ren, Weibo,et al. Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions[J]. JOURNAL OF PLANT BIOLOGY,2016,59(4):336-346.
APA	Li, Lingyu.,Yang, Haomeng.,Ren, Weibo.,Liu, Bei.,Cheng, Dongmei.,...&Huang, Fang.(2016).Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions.JOURNAL OF PLANT BIOLOGY,59(4),336-346.
MLA	Li, Lingyu,et al."Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions".JOURNAL OF PLANT BIOLOGY 59.4(2016):336-346.

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