Comparative proteomics of leaves found at different stem positions of maize seedlings

doi:10.1016/j.jplph.2016.03.022

IB-CAS > 中科院光生物学重点实验室

	Comparative proteomics of leaves found at different stem positions of maize seedlings
	Chen, Yi-Bo ; Wang, Dan ; Ge, Xuan-Liang ; Zhao, Biligen-Gaowa ; Wang, Xu-Chu ; Wang, Bai-Chen
	2016
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	198 页码:116-128
摘要	To better understand the roles of leaves at different stem positions during plant development, we measured the physiological properties of leaves 1-4 on maize seedling stems, and performed a proteomics study to investigate the differences in protein expression in the four leaves using two-dimensional difference gel electrophoresis and tandem mass spectrometry in conjunction with database searching. A total of 167 significantly differentially expressed protein spots were found and identified. Of these, 35% are involved in photosynthesis. By further analysis of the data, we speculated that in leaf 1 the seedling has started to transition from a heterotroph to an autotroph, development of leaf 2 is the time at which the seedling fully transitions from a heterotroph to an autotroph, and leaf maturity was reached only with fully expanded leaves 3 and 4, although there were still some protein expression differences in the two leaves. These results suggest that the different leaves make different contributions to maize seedling growth via modulation of the expression of the photosynthetic proteins. Together, these results provide insight into the roles of the different maize leaves as the plant develops from a heterotroph to an autotroph. (C) 2016 Elsevier GmbH. All rights reserved.
关键词	C4 photosynthesis Leaf position Proteomics Two-dimensional difference gel electrophoresis Tandem mass spectrometry
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2016.03.022
收录类别	SCI
语种	英语
WOS关键词	I QUANTUM YIELD ; C-4 PHOTOSYNTHESIS ; QUANTITATIVE PROTEOMICS ; LEAF DEVELOPMENT ; BINDING ; ARABIDOPSIS ; PROTEINS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000376820600012
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
文献子类	Article
出版地	JENA
EISSN	1618-1328
资助机构	General Program of National Natural Science of China [31471506] ; State Key Program of National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31030017] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA08010206.5]
作者邮箱	xchwanghainan@163.com ; wangbc@ibcas.ac.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25305
专题	中科院光生物学重点实验室
作者单位	1.[Chen, Yi-Bo 2.Zhao, Biligen-Gaowa 3.Chinese Acad Sci, Key Lab Photobiol, Photosynth Res Ctr, Inst Bot, Beijing, Peoples R China 4.[Wang, Dan 5.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou, Hainan, Peoples R China 6.Heilongjiang Acad Agr Sci, Inst Cultivat & Tillage, Haerbin, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yi-Bo,Wang, Dan,Ge, Xuan-Liang,et al. Comparative proteomics of leaves found at different stem positions of maize seedlings[J]. JOURNAL OF PLANT PHYSIOLOGY,2016,198:116-128.
APA	Chen, Yi-Bo,Wang, Dan,Ge, Xuan-Liang,Zhao, Biligen-Gaowa,Wang, Xu-Chu,&Wang, Bai-Chen.(2016).Comparative proteomics of leaves found at different stem positions of maize seedlings.JOURNAL OF PLANT PHYSIOLOGY,198,116-128.
MLA	Chen, Yi-Bo,et al."Comparative proteomics of leaves found at different stem positions of maize seedlings".JOURNAL OF PLANT PHYSIOLOGY 198(2016):116-128.

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