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NdhV subunit regulates the activity of type-1 NAD(P)H dehydrogenase under high light conditions in cyanobacterium Synechocystis sp PCC 6803 | |
Chen, Xin1; He, Zhihui; Xu, Min; Peng, Lianwei2; Mi, Hualing | |
2016 | |
发表期刊 | SCIENTIFIC REPORTS |
ISSN | 2045-2322 |
卷号 | 6 |
摘要 | The cyanobacterial NAD(P)H dehydrogenase (NDH-1) complexes play crucial roles in variety of bioenergetic reactions. However, the regulative mechanism of NDH-1 under stressed conditions is still unclear. In this study, we detected that the NDH-1 activity is partially impaired, but the accumulation of NDH-1 complexes was little affected in the NdhV deleted mutant (Delta ndhV) at low light in cyanobacterium Synechocystis sp. PCC 6803. Delta ndhV grew normally at low light but slowly at high light under inorganic carbon limitation conditions (low pH or low CO2), meanwhile the activity of CO2 uptake was evidently lowered than wild type even at pH 8.0. The accumulation of NdhV in thylakoids strictly relies on the presence of the hydrophilic subcomplex of NDH-1. Furthermore, NdhV was co-located with hydrophilic subunits of NDH-1 loosely associated with the NDH-1L, NDH-1MS' and NDH-1M complexes. The level of the NdhV was significantly increased at high light and deletion of NdhV suppressed the up-regulation of NDH-1 activity, causing the lowered the photosynthetic oxygen evolution at pH 6.5 and high light. These data indicate that NdhV is an intrinsic subunit of hydrophilic subcomplex of NDH-1, required for efficient operation of cyclic electron transport around photosystem I and CO2 uptake at high lights. |
学科领域 | Multidisciplinary Sciences |
DOI | 10.1038/srep28361 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | CYCLIC ELECTRON-TRANSPORT ; PYRIDINE-NUCLEOTIDE DEHYDROGENASE ; RESPIRATORY COMPLEX I ; DOMAIN-LIKE FOLD ; NADPH DEHYDROGENASE ; PHOTOSYSTEM-I ; SP PCC-6803 ; CO2 UPTAKE ; THERMOSYNECHOCOCCUS-ELONGATUS ; INTERSYSTEM CHAIN |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000378225300002 |
出版者 | NATURE PUBLISHING GROUP |
文献子类 | Article |
出版地 | LONDON |
资助机构 | State Key Basic Research and Development Plan 973National Basic Research Program of China [2013CB127005, 2015CB150104] ; National Natural Scientific Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270286, 31470338] ; Shanghai Science Foundation [13DJ1400102] |
作者邮箱 | hlmi@sibs.ac.cn |
作品OA属性 | Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25308 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, 300 Fenglin Rd, Shanghai 200032, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Xin,He, Zhihui,Xu, Min,et al. NdhV subunit regulates the activity of type-1 NAD(P)H dehydrogenase under high light conditions in cyanobacterium Synechocystis sp PCC 6803[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Chen, Xin,He, Zhihui,Xu, Min,Peng, Lianwei,&Mi, Hualing.(2016).NdhV subunit regulates the activity of type-1 NAD(P)H dehydrogenase under high light conditions in cyanobacterium Synechocystis sp PCC 6803.SCIENTIFIC REPORTS,6. |
MLA | Chen, Xin,et al."NdhV subunit regulates the activity of type-1 NAD(P)H dehydrogenase under high light conditions in cyanobacterium Synechocystis sp PCC 6803".SCIENTIFIC REPORTS 6(2016). |
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