The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins

doi:10.1007/s11103-016-0466-7

IB-CAS > 中科院光生物学重点实验室

	The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins
	Chao, Qing ; Gao, Zhi-fang ; Wang, Yue-feng ; Li, Zhe 1; Huang, Xia-he ; Wang, Ying-chun ; Mei, Ying-chang ; Zhao, Biligen-gaowa ; Li, Liang ; Jiang, Yu-bo ; Wang, Bai-chen
	2016
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	91 期号:3 页码:287-304
摘要	Maize is unique since it is both monoecious and diclinous (separate male and female flowers on the same plant). We investigated the proteome and phosphoproteome of maize pollen containing modified proteins and here we provide a comprehensive pollen proteome and phosphoproteome which contain 100,990 peptides from 6750 proteins and 5292 phosphorylated sites corresponding to 2257 maize phosphoproteins, respectively. Interestingly, among the total 27 overrepresented phosphosite motifs we identified here, 11 were novel motifs, which suggested different modification mechanisms in plants compared to those of animals. Enrichment analysis of pollen phosphoproteins showed that pathways including DNA synthesis/chromatin structure, regulation of RNA transcription, protein modification, cell organization, signal transduction, cell cycle, vesicle transport, transport of ions and metabolisms, which were involved in pollen development, the following germination and pollen tube growth, were regulated by phosphorylation. In this study, we also found 430 kinases and 105 phosphatases in the maize pollen phosphoproteome, among which calcium dependent protein kinases (CDPKs), leucine rich repeat kinase, SNF1 related protein kinases and MAPK family proteins were heavily enriched and further analyzed. From our research, we also uncovered hundreds of male sterility-associated proteins and phosphoproteins that might influence maize productivity and serve as targets for hybrid maize seed production. At last, a putative complex signaling pathway involving CDPKs, MAPKs, ubiquitin ligases and multiple fertility proteins was constructed. Overall, our data provides new insight for further investigation of protein phosphorylation status in mature maize pollen and construction of maize male sterile mutants in the future.
关键词	Maize pollen Proteome Phosphoproteome Motif Kinase Male sterile
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-016-0466-7
收录类别	SCI
语种	英语
WOS关键词	MALE GAMETOPHYTE DEVELOPMENT ; TUBE TIP GROWTH ; MALE-STERILITY ; POLARIZED GROWTH ; CELL-DIVISION ; K+ CHANNEL ; ZEA-MAYS ; ARABIDOPSIS ; PHOSPHORYLATION ; GERMINATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000377025200005
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National High-Tech Research and Development Program of ChinaNational High Technology Research and Development Program of China [2012AA10A307]
作者邮箱	wangbc@ibcas.ac.cn
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25311
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 4.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China
推荐引用方式 GB/T 7714	Chao, Qing,Gao, Zhi-fang,Wang, Yue-feng,et al. The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins[J]. PLANT MOLECULAR BIOLOGY,2016,91(3):287-304.
APA	Chao, Qing.,Gao, Zhi-fang.,Wang, Yue-feng.,Li, Zhe.,Huang, Xia-he.,...&Wang, Bai-chen.(2016).The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins.PLANT MOLECULAR BIOLOGY,91(3),287-304.
MLA	Chao, Qing,et al."The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins".PLANT MOLECULAR BIOLOGY 91.3(2016):287-304.

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