Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana

doi:10.1107/S2053230X16007263

IB-CAS > 中科院光生物学重点实验室

	Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana
	Song, Yingxian; Pu, Hua; Jiang, Tian; Zhang, Lixin; Ouyang, Min
	2016
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷号	72 页码:448-456
摘要	Glutamate-1-semialdehyde-2,1-aminomutase (GSAM) catalyzes the isomerization of glutamate-1-semialdehyde (GSA) to 5-aminolevulinate (ALA) and is distributed in archaea, most bacteria and plants. Although structures of GSAM from archaea and bacteria have been resolved, a GSAM structure from a higher plant is not available, preventing further structure-function analysis. Here, the structure of GSAM from Arabidopsis thaliana (AtGSA1) obtained by X-ray crystallography is reported at 1.25 angstrom resolution. AtGSA1 forms an asymmetric dimer and displays asymmetry in cofactor binding as well as in the gating-loop orientation, which is consistent with previously reported Synechococcus GSAM structures. While one monomer binds PMP with the gating loop fixed in the open state, the other monomer binds either PMP or PLP and the gating loop is ready to close. The data also reveal the mobility of residues Gly163, Ser164 and Gly165, which are important for reorientation of the gating loop. Furthermore, the asymmetry of the AtGSA1 structure supports the previously proposed negative cooperativity between monomers of GSAM.
关键词	X-ray crystallography asymmetry pyridoxamine 5 '-phosphate pyridoxal 5 '-phosphate gating loop glutamate-1-semialdehyde-2,1-aminomutase Arabidopsis thaliana
学科领域	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
DOI	10.1107/S2053230X16007263
收录类别	SCI
语种	英语
WOS关键词	GLUTAMYL-TRANSFER-RNA ; NEGATIVE COOPERATIVITY ; 1-SEMIALDEHYDE AMINOTRANSFERASE ; SEMIALDEHYDE AMINOTRANSFERASE ; CHLOROPHYLL BIOSYNTHESIS ; ENZYME ; 2,1-AMINOMUTASE ; SYNECHOCOCCUS ; MECHANISM ; REDUCTASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000380288600005
出版者	INT UNION CRYSTALLOGRAPHY
文献子类	Article
出版地	CHESTER
EISSN	2053-230X
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471267]
作者邮箱	ouyangmin@ibcas.ac.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25313
专题	中科院光生物学重点实验室
作者单位	Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Song, Yingxian,Pu, Hua,Jiang, Tian,et al. Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2016,72:448-456.
APA	Song, Yingxian,Pu, Hua,Jiang, Tian,Zhang, Lixin,&Ouyang, Min.(2016).Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,72,448-456.
MLA	Song, Yingxian,et al."Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 72(2016):448-456.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Song-2016-Crystal st（2526KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文