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An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity | |
Lin, Xiao-Li; Niu, De; Hu, Zi-Liang; Kim, Dae Heon1; Jin, Yin Hua; Cai, Bin; Liu, Peng5; Miura, Kenji2; Yun, Dae-Jin; Kim, Woe-Yeon; Lin, Rongcheng4; Jin, Jing Bo | |
2016 | |
发表期刊 | PLOS GENETICS |
ISSN | 1553-7404 |
卷号 | 12期号:4 |
摘要 | COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), a ubiquitin E3 ligase, is a central negative regulator of photomorphogenesis. However, how COP1 activity is regulated by post-translational modifications remains largely unknown. Here we show that SUMO (small ubiquitin-like modifier) modification enhances COP1 activity. Loss-of-function siz1 mutant seedlings exhibit a weak constitutive photomorphogenic phenotype. SIZ1 physically interacts with COP1 and mediates the sumoylation of COP1. A K193R substitution in COP1 blocks its SUMO modification and reduces COP1 activity in vitro and in planta. Consistently, COP1 activity is reduced in siz1 and the level of HY5, a COP1 target protein, is increased in siz1. Sumoylated COP1 may exhibits higher transubiquitination activity than does non-sumoylated COP1, but SIZ1-mediated SUMO modification does not affect COP1 dimerization, COP1-HY5 interaction, and nuclear accumulation of COP1. Interestingly, prolonged light exposure reduces the sumoylation level of COP1, and COP1 mediates the ubiquitination and degradation of SIZ1. These regulatory mechanisms may maintain the homeostasis of COP1 activity, ensuing proper photomorphogenic development in changing light environment. Our genetic and biochemical studies identify a function for SIZ1 in photomorphogenesis and reveal a novel SUMO-regulated ubiquitin ligase, COP1, in plants. |
学科领域 | Genetics & Heredity |
DOI | 10.1371/journal.pgen.1006016 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | LIGHT-DEPENDENT INTERACTION ; BLUE-LIGHT ; CONSTITUTIVE PHOTOMORPHOGENIC1 ; ELONGATED HYPOCOTYL5 ; SEEDLING DEVELOPMENT ; GENE-EXPRESSION ; CRYPTOCHROME 1 ; PROTEIN ; SUMOYLATION ; PHYTOCHROME |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000375231900046 |
出版者 | PUBLIC LIBRARY SCIENCE |
文献子类 | Article |
出版地 | SAN FRANCISCO |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170170, 31471363] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB114302] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105] |
作者邮箱 | jinjb@ibcas.ac.cn |
作品OA属性 | Green Published, Green Submitted, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25319 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Sunchon Natl Univ, Dept Biol, Sunchon, South Korea 3.Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan 4.Gyeongsang Natl Univ, PMBBRC & IALS, Div Appl Life Sci BK21Plus, Jinju, South Korea 5.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 6.King Abdullah Univ Sci & Technol, Div Biol Environm Sci & Engn, Thuwal, Saudi Arabia |
推荐引用方式 GB/T 7714 | Lin, Xiao-Li,Niu, De,Hu, Zi-Liang,et al. An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity[J]. PLOS GENETICS,2016,12(4). |
APA | Lin, Xiao-Li.,Niu, De.,Hu, Zi-Liang.,Kim, Dae Heon.,Jin, Yin Hua.,...&Jin, Jing Bo.(2016).An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity.PLOS GENETICS,12(4). |
MLA | Lin, Xiao-Li,et al."An Arabidopsis SUMO E3 Ligase, SIZ1, Negatively Regulates Photomorphogenesis by Promoting COP1 Activity".PLOS GENETICS 12.4(2016). |
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