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Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis
Xu, Yangyang1; Wu, Hanying; Zhao, Mingming1; Wu, Wang1; Xu, Yinong; Gu, Dan
2016
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume17Issue:4
AbstractSHINE (SHN/WIN) clade proteins, transcription factors of the plant-specific APETALA 2/ethylene-responsive element binding factor (AP2/ERF) family, have been proven to be involved in wax and cutin biosynthesis. Glycine max is an important economic crop, but its molecular mechanism of wax biosynthesis is rarely characterized. In this study, 10 homologs of Arabidopsis SHN genes were identified from soybean. These homologs were different in gene structures and organ expression patterns. Constitutive expression of each of the soybean SHN genes in Arabidopsis led to different leaf phenotypes, as well as different levels of glossiness on leaf surfaces. Overexpression of GmSHN1 and GmSHN9 in Arabidopsis exhibited 7.8-fold and 9.9-fold up-regulation of leaf cuticle wax productions, respectively. C31 and C29 alkanes contributed most to the increased wax contents. Total cutin contents of leaves were increased 11.4-fold in GmSHN1 overexpressors and 5.7-fold in GmSHN9 overexpressors, mainly through increasing C16:0 di-OH and dioic acids. GmSHN1 and GmSHN9 also altered leaf cuticle membrane ultrastructure and increased water loss rate in transgenic Arabidopsis plants. Transcript levels of many wax and cutin biosynthesis and leaf development related genes were altered in GmSHN1 and GmSHN9 overexpressors. Overall, these results suggest that GmSHN1 and GmSHN9 may differentially regulate the leaf development process as well as wax and cutin biosynthesis.
Keywordwax cutin SHN/WIN AP2/ERF soybean overexpression transcription factor leaf development
Subject AreaBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI10.3390/ijms17040587
Indexed BySCI
Language英语
WOS KeywordAGROBACTERIUM-MEDIATED TRANSFORMATION ; BETA-KETOACYL-COENZYME ; FATTY ACYL-COENZYME ; SYNTHETASE 1 LACS1 ; DROUGHT TOLERANCE ; ALKANE BIOSYNTHESIS ; FUNCTIONAL-ANALYSIS ; BARRIER PROPERTIES ; GENE-EXPRESSION ; ACCUMULATION
WOS Research AreaScience Citation Index Expanded (SCI-EXPANDED)
WOS IDWOS:000374585300165
PublisherMDPI
SubtypeArticle
Publication PlaceBASEL
EISSN1422-0067
Funding OrganizationNational Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31501381, 31370386]
Corresponding Author Emailyyxu@ibcas.ac.cn ; whywu@ibcas.ac.cn ; zhaomm@bioyong.com ; wuwang19@163.com ; yinongxu@ibcas.ac.cn ; dangu@ibcas.ac.cn
OAgold, Green Published, Green Submitted
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/25320
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xu, Yangyang,Wu, Hanying,Zhao, Mingming,et al. Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(4).
APA Xu, Yangyang,Wu, Hanying,Zhao, Mingming,Wu, Wang,Xu, Yinong,&Gu, Dan.(2016).Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(4).
MLA Xu, Yangyang,et al."Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.4(2016).
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