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| Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis | |
| Xu, Yangyang1; Wu, Hanying; Zhao, Mingming1; Wu, Wang1; Xu, Yinong; Gu, Dan | |
| 2016 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| Volume | 17Issue:4 |
| Abstract | SHINE (SHN/WIN) clade proteins, transcription factors of the plant-specific APETALA 2/ethylene-responsive element binding factor (AP2/ERF) family, have been proven to be involved in wax and cutin biosynthesis. Glycine max is an important economic crop, but its molecular mechanism of wax biosynthesis is rarely characterized. In this study, 10 homologs of Arabidopsis SHN genes were identified from soybean. These homologs were different in gene structures and organ expression patterns. Constitutive expression of each of the soybean SHN genes in Arabidopsis led to different leaf phenotypes, as well as different levels of glossiness on leaf surfaces. Overexpression of GmSHN1 and GmSHN9 in Arabidopsis exhibited 7.8-fold and 9.9-fold up-regulation of leaf cuticle wax productions, respectively. C31 and C29 alkanes contributed most to the increased wax contents. Total cutin contents of leaves were increased 11.4-fold in GmSHN1 overexpressors and 5.7-fold in GmSHN9 overexpressors, mainly through increasing C16:0 di-OH and dioic acids. GmSHN1 and GmSHN9 also altered leaf cuticle membrane ultrastructure and increased water loss rate in transgenic Arabidopsis plants. Transcript levels of many wax and cutin biosynthesis and leaf development related genes were altered in GmSHN1 and GmSHN9 overexpressors. Overall, these results suggest that GmSHN1 and GmSHN9 may differentially regulate the leaf development process as well as wax and cutin biosynthesis. |
| Keyword | wax cutin SHN/WIN AP2/ERF soybean overexpression transcription factor leaf development |
| Subject Area | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms17040587 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | AGROBACTERIUM-MEDIATED TRANSFORMATION ; BETA-KETOACYL-COENZYME ; FATTY ACYL-COENZYME ; SYNTHETASE 1 LACS1 ; DROUGHT TOLERANCE ; ALKANE BIOSYNTHESIS ; FUNCTIONAL-ANALYSIS ; BARRIER PROPERTIES ; GENE-EXPRESSION ; ACCUMULATION |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000374585300165 |
| Publisher | MDPI |
| Subtype | Article |
| Publication Place | BASEL |
| EISSN | 1422-0067 |
| Funding Organization | National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31501381, 31370386] |
| Corresponding Author Email | yyxu@ibcas.ac.cn ; whywu@ibcas.ac.cn ; zhaomm@bioyong.com ; wuwang19@163.com ; yinongxu@ibcas.ac.cn ; dangu@ibcas.ac.cn |
| OA | gold, Green Published, Green Submitted |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25320 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Yangyang,Wu, Hanying,Zhao, Mingming,et al. Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(4). |
| APA | Xu, Yangyang,Wu, Hanying,Zhao, Mingming,Wu, Wang,Xu, Yinong,&Gu, Dan.(2016).Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(4). |
| MLA | Xu, Yangyang,et al."Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.4(2016). |
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| File Name/Size | DocType | Version | Access | License | ||
| Xu-2016-Overexpressi（11185KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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